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| 文献 | ||
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| 著者 | Yang Z., Liu L., Sun L., Yu P., Zhang P., Abbas A., Xiang X., Wu W., Zhang Y., Cao L., Cheng S. | |
| タイトル | osms1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice. | |
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Abstract: osms1 functions as a transcriptional activator and interacts with known tapetal regulatory factors through its plant homeodomain (PHD) regulating tapetal programmed cell death (PCD) and pollen exine formation in rice. The tapetum, a hallmark tissue in the stamen, undergoes degradation triggered by PCD during post-meiotic anther development. This degradation process is indispensable for anther cuticle and pollen exine formation. Previous study has shown that PTC1 plays a critical role in the regulation of tapetal PCD. However, it remained unclear how this occurs. To further investigate the role of this gene in rice, we used CRISPR/Cas9 system to generate the homozygous mutant named as osms1, which showed complete male sterility with slightly yellow and small anthers, as well as invisible pollen grains. In addition, cytological observation revealed delayed tapetal PCD, defective pollen exine formation and a lack of DNA fragmentation according to a TUNEL analysis in the anthers of osms1 mutant. osms1, which encodes a PHD finger protein, was located in the nucleus of rice protoplasts and functioned as a transcription factor with transcriptional activation activity. Y2H and BiFC assays demonstrated that osms1 can interact with OsMADS15 and TDR INTERACTING PROTEIN2 (TIP2). It has been reported that TIP2 coordinated with TDR to modulate the expression of EAT1 and further regulated tapetal PCD in rice. Results of qPCR suggested that the expression of the genes associated with tapetal PCD and pollen wall biosynthesis, such as EAT1, AP37, AP25, OsC6 and OsC4, were significantly reduced in osms1 mutant. Taken together, our results demonstrate that the interaction of osms1 with known tapetal regulatory factors through its PHD finger regulates tapetal PCD and pollen exine formation in rice. |
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| 掲載誌 | Plant Mol. Biol. | |
| Country | China | |
| 刊数 | 99(1-2) | |
| ページ | 175-191 | |
| 発刊年 | 2019 | |
| PubMed ID | 30610522 | |
| PubMed Central ID | - | |
| DOI | 10.1007/s11103-018-0811-0 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | AP37 BHLH142 C6 CP1 CYP703A3 CYP704B2 EAT1 GAMYB OSC4 PTC1 RADC1 RAP1A TDR | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
