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Reference Detail
| Reference | ||
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| Author | Chandran V., Wang H., Gao F., Cao X.L., Chen Y.P., Li G.B., Zhu Y., Yang X.M., Zhang L.L., Zhao Z.X., Zhao J.H., Wang Y.G., Li S., Fan J., Li Y., Zhao J.Q., Li S.Q., Wang W.M. | |
| Title | MiR396-<i>OsGRF</i>s Module Balances Growth and Rice blast disease-Resistance. | |
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Abstract: Fitness cost is a common phenomenon in rice blast disease-resistance breeding. MiR396 is a highly conserved microRNA (miRNA) family targeting <i>Growth Regulating Factor</i> (<i>OsGRF</i>) genes. Mutation at the target site of MiR396 in certain <i>OsGRF</i> gene or blocking MiR396 expression leads to increased grain yield. Here we demonstrated that fitness cost can be trade-off in MiR396-<i>OsGRF</i>s module via balancing growth and immunity against the blast fungus. The accumulation of MiR396 isoforms was significantly increased in a susceptible accession, but fluctuated in a resistant accession upon infection of <i>Magnaporthe oryzae</i>. The transgenic lines over-expressing different MiR396 isoforms were highly susceptible to <i>M. oryzae</i>. In contrast, overexpressing target mimicry of MiR396 to block its function led to enhanced resistance to <i>M. oryzae</i> in addition to improved yield traits. Moreover, transgenic plants overexpressing <i>OsGRF6</i>, <i>OsGRF7</i>, <i>OsGRF8</i>, and <i>OsGRF9</i> exhibited enhanced resistance to <i>M. oryzae</i>, but showed different alteration of growth. While overexpression of <i>OsGRF7</i> led to defects in growth, overexpression of <i>OsGRF6</i>, <i>OsGRF8</i>, and <i>OsGRF9</i> resulted in better or no significant change of yield traits. Collectively, our results indicate that MiR396 negatively regulates rice blast disease- resistance via suppressing multiple <i>OsGRF</i>s, which in turn differentially control growth and yield. Therefore, MiR396-<i>OsGRFs</i> could be a potential module to demolish fitness cost in rice blast disease-resistance breeding. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 9 | |
| Pages | 1999 | |
| Year | 2018 | |
| PubMed ID | 30693011 | |
| PubMed Central ID | 6339958 | |
| DOI | 10.3389/fpls.2018.01999 | |
| URL | - | |
| Relation | ||
| Gene | GRF11 GRF2 GRF3 GRF5 GRF6 GRF7 GRF8 GRF9 GS2 MIR396A MIR396B MIR396C MIR396D MIR396E MIR396F MIR396G MIR396H | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
