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Author El-Esawi MA., Alayafi A.A.
Title Overexpression of Rice <i>Rab7</i> Gene Improves Drought and heat tolerance and Increases grain yield in Rice (<i>Oryza sativa</i> L.).
Abstract:
Rab family proteins play a crucial role in plant developmental processes and tolerance to environmental stresses. The current study investigated whether rice <i>Rab7</i> (<i>OsRab7</i>) overexpression could improve rice tolerance to drought and heat stress conditions. The <i>OsRab7</i> gene was cloned and transformed into rice plants. The survival rate, relative water content, chlorophyll content, gas-exchange characteristics, soluble protein content, soluble sugar content, proline content, and activities of antioxidant enzymes (CAT, SOD, APX, POD) of the transgenic rice lines were significantly higher than that of the wild-type. In contrast, the levels of hydrogen peroxide, electrolyte leakage, and malondialdehyde of the transgenic lines were significantly reduced when compared to wild-type. Furthermore, the expression of four genes encoding reactive oxygen species (ROS)-scavenging enzymes (<i>OsCATA</i>, <i>OsCATB</i>, <i>OsAPX2</i>, <i>OsSOD-Cu/Zn</i>) and eight genes conferring abiotic stress tolerance (<i>OsLEA3</i>, <i>OsRD29A</i>, <i>OsSNAC1</i>, <i>OsSNAC2</i>, <i>OsDREB2A</i>, <i>OsDREB2B</i>, <i>OsRAB16A</i>, <i>OsRAB16C</i>) was significantly up-regulated in the transformed rice lines as compared to their expression in wild-type. <i>OsRab7</i> overexpression also increased grain yield in rice. Taken together, the current study indicates that the <i>OsRab7</i> gene improves grain yield and enhances drought and heat tolerance in transgenic rice by modulating osmolytes, antioxidants and abiotic stress-responsive genes expression. Therefore, <i>OsRab7</i> gene could be exploited as a promising candidate for improving rice grain yield and stress tolerance.
Journal Genes (Basel)
Country Egypt
Volume 10(1)
Pages
Year 2019
PubMed ID 30658457
PubMed Central ID 6357162
DOI 10.3390/genes10010056
URL -
Relation
Gene CATA CATB DREB2A ERF42 LEA3-1 NAC6 RAB16A RAB16C _ _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
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