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Reference Detail
| Reference | ||
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| Author | Zha K., Xie H., Ge M., Wang Z., Wang Y., Si W., Gu L. | |
| Title | Expression of Maize MADS Transcription Factor <i>ZmES22</i> Negatively Modulates Starch Accumulation in Rice Endosperm. | |
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Abstract: As major component in cereals grains, starch has been one of the most important carbohydrate consumed by a majority of world's population. However, the molecular mechanism for regulation of biosynthesis of starch remains elusive. In the present study, <i>ZmES22</i>, encoding a MADS-type transcription factor, was modestly characterized from maize inbred line B73. <i>ZmES22</i> exhibited high expression level in endosperm at 10 days after pollination (DAP) and peaked in endosperm at 20 DAP, indicating that <i>ZmES22</i> was preferentially expressed in maize endosperm during active starch synthesis. Transient expression of <i>ZmES22</i> in tobacco leaf revealed that ZmES22 protein located in nucleus. No transactivation activity could be detected for ZmES22 protein via yeast one-hybrid assay. Transformation of overexpressing plasmid 35S::<i>ZmES22</i> into rice remarkedly reduced 1000-grain weight as well as the total starch content, while the soluble sugar was significantly higher in transgenic rice lines. Moreover, overexpressing <i>ZmES22</i> reduced fractions of long branched starch. Scanning electron microscopy images of transverse sections of rice grains revealed that altered expression of <i>ZmES22</i> also changed the morphology of starch granule from densely packed, polyhedral starch granules into loosely packed, spherical granules with larger spaces. Furthermore, RNA-seq results indicated that overexpressing <i>ZmES22</i> could significantly influence mRNA expression levels of numerous key regulatory genes in starch synthesis pathway. Y1H assay illustrated that ZmES22 protein could bind to the promoter region of <i>OsGIF1</i> and downregulate its mRNA expression during rice grain filling stages. These findings suggest that <i>ZmES22</i> was a novel regulator during starch synthesis process in rice endosperm. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 20(3) | |
| Pages | ||
| Year | 2019 | |
| PubMed ID | 30678069 | |
| PubMed Central ID | 6387075 | |
| DOI | 10.3390/ijms20030483 | |
| URL | - | |
| Relation | ||
| Gene | GIF1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
