Tools - Reference - Detail

Reference Detail

Reference
Author Fang M., Zhou Z., Zhou X., Yang H., Li M., Li H.
Title Overexpression of <i>OsFTL10</i> induces early flowering and improves drought tolerance in <i>Oryza sativa</i> L.
Abstract:
Flowering time control is critically important for the reproductive accomplishment of higher plants as floral transition can be affected by both environmental and endogenous signals. Flowering Locus T-like (<i>FTL</i>) genes are major genetic determinants of flowering in plants. In rice, 13 <i>OsFTL</i> genes have been annotated in the genome and amongst them, <i>Hd3a</i> (<i>OsFTL2</i>) and <i>RFT1</i> (<i>OsFTL3</i>) have been studied extensively and their functions are confirmed as central florigens that control rice flowering under short day and long day environment, respectively. In this report, a rice <i>OsFTL</i> gene, <i>OsFTL10</i>, was characterized, and its function on flowering and abiotic stress was investigated. The expression level of <i>OsFTL10</i> was high in young seedlings and shown to be induced by GA3 and drought stress. Overexpression of <i>OsFTL10</i> resulted in earlier flowering in rice plants by up to 2 weeks, through up-regulation of the downstream gene <i>OsMADS15</i>. <i>OsFTL10</i> also regulated <i>Ehd1</i> and <i>OsMADS51</i> through a feedback mechanism. The OsFTL10 protein was also detected in both nucleus and cytoplasm. Furthermore, yeast two hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) results show that OsFTL10 could interact with multiple 14-3-3s, suggesting that OsFTL10 might function in a similar way to Hd3a in promoting rice flowering by forming a FAC complex with 14-3-3, and OsFD1. Further experiments revealed that constitutive expression of <i>OsFTL10</i> improved the drought tolerance of transgenic plants by stimulating the expression of drought responsive genes. These results suggest that rice <i>FTL</i> genes might function in flowering promotion and responses to environmental signals.
Journal PeerJ
Country China
Volume 7
Pages e6422
Year 2019
PubMed ID 30783573
PubMed Central ID 6376957
DOI 10.7717/peerj.6422
URL -
Relation
Gene BZIP23 DREB2A EF1 FTL1 FTL10 GF14A GF14B GF14C GF14D GID2 HD3A MADS51 RAP1A RAP1B RFT1 _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase