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Author Andrade L.M., Peixoto-Junior RF., Ribeiro R.V., Nóbile PM., Brito M.S., Marchiori P.E.R., Carlin S.D., Martins A.P.B., Goldman M.H.S., Llerena J.P.P., Fregonesi C., Perecin D., Nebó JFCO., Figueira A., Benatti T.R., da Silva J., Mazzafera P., Creste S.
Title Biomass Accumulation and Cell Wall Structure of Rice Plants Overexpressing a Dirigent-Jacalin of Sugarcane (<i>ShDJ</i>) Under Varying Conditions of Water Availability.
A sugarcane gene encoding a <i>dirigent-jacalin</i>, <i>ShDJ</i>, was induced under drought stress. To elucidate its biological function, we integrated a <i>ShDJ</i>-overexpression construction into the rice Nipponbare genome via <i>Agrobacterium</i>-mediated transformation. Two transgenic lines with a single copy gene in T0 were selected and evaluated in both the T1 and T4 generations. Transgenic lines had drastically improved survival rate under water deficit conditions, at rates close to 100%, while WT did not survive. Besides, transgenic lines had improved biomass production and higher tillering under water deficit conditions compared with WT plants. Reduced pectin and hemicellulose contents were observed in transgenic lines compared with wild-type plants under both well-watered and water deficit conditions, whereas cellulose content was unchanged in line #17 and reduced in line #29 under conditions of low water availability. Changes in lignin content under water deficit were only observed in line #17. However, improvements in saccharification were found in both transgenic lines along with changes in the expression of <i>OsNTS1/2</i> and <i>OsMYB58/63</i> secondary cell wall biosynthesis genes. <i>ShDJ</i>-overexpression up-regulated the expression of the <i>OsbZIP23</i>, <i>OsGRAS23</i>, <i>OsP5CS</i>, and <i>OsLea3</i> genes in rice stems under well-watered conditions. Taken together, our data suggest that <i>ShDJ</i> has the potential for improving drought tolerance, plant biomass accumulation, and saccharification efficiency.
Journal Front Plant Sci
Country Brazil
Volume 10
Pages 65
Year 2019
PubMed ID 30815002
PubMed Central ID 6381051
DOI 10.3389/fpls.2019.00065
Gene BZIP23 GRAS23 LEA3-1 NAC29 P5CS _
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
Stages in Each Organ
- Muant Lines (Gene)
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -