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| 文献 | ||
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| 著者 | Sun L., Xiang X., Yang Z., Yu P., Wen X., Wang H., Abbas A., Muhammad Khan R., Zhang Y., Cheng S., Cao L. | |
| タイトル | <i>OsGPAT3</i> Plays a Critical Role in anther wall Programmed Cell Death and pollen Development in Rice. | |
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Abstract: In flowering plants, ideal male reproductive development requires the systematic coordination of various processes, in which timely differentiation and degradation of the anther wall, especially the tapetum, is essential for both pollen formation and anther dehiscence. Here, we show that <i>OsGPAT3</i>, a conserved glycerol-3-phosphate acyltransferase gene, plays a critical role in regulating anther wall degradation and pollen exine formation. The <i>gpat3-2</i> mutant had defective synthesis of Ubisch bodies, delayed programmed cell death (PCD) of the inner three anther layers, and abnormal degradation of micropores/pollen grains, resulting in failure of pollen maturation and complete male sterility. Complementation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) experiments demonstrated that <i>OsGPAT3</i> is responsible for the male sterility phenotype. Furthermore, the expression level of tapetal PCD-related and nutrient metabolism-related genes changed significantly in the <i>gpat3-2</i> anthers. Based on these genetic and cytological analyses, <i>OsGPAT3</i> is proposed to coordinate the differentiation and degradation of the anther wall and pollen grains in addition to regulating lipid biosynthesis. This study provides insights for understanding the function of <i>GPATs</i> in regulating rice male reproductive development, and also lays a theoretical basis for hybrid rice breeding. |
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| 掲載誌 | Int J Mol Sci | |
| Country | China | |
| 刊数 | 19(12) | |
| ページ | ||
| 発刊年 | 2018 | |
| PubMed ID | 30545137 | |
| PubMed Central ID | 6321289 | |
| DOI | 10.3390/ijms19124017 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | ABCG15 ACS9 AP37 BHLH142 BZIP74 C6 CP1 CYP703A3 CYP704B2 DEX1 DPW2 EAT1 GAMYB GPAT3 MADS24 MS2 MSP1 MTR1 NP1 PSBS1 RADC1 RAG TDR WDA1 _ _ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
