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Author Um T.Y., Lee H.Y., Lee S., Chang S.H., Chung P.J., Oh K.B., Kim J.K., Jang G., Choi Y.D.
Title JASMONATE ZIM-DOMAIN PROTEIN 9 Interacts With Slender Rice 1 to Mediate the Antagonistic Interaction Between Jasmonic and Gibberellic Acid Signals in Rice.
Abstract:
The jasmonic acid (JA) and gibberellic acid (GA) signaling pathways interact to coordinate stress responses and developmental processes. This coordination affects plant growth and yield, and is mediated by interactions between the repressors of each pathway, the JASMONATE ZIM-DOMAIN PROTEIN (JAZ) and DELLA proteins. In this study we attempted to identify rice (<i>Oryza sativa</i>) JAZs that interact with rice DELLAs such as Slender Rice 1 (SLR1). Analysis of protein-protein interactions showed that OsJAZ8 and OsJAZ9 interact with SLR1; OsJAZ9 also interacted with the SLR1-LIKE (SLRL) protein SLRL2. Based on this broader interaction, we explored the function of OsJAZ9 in JA and GA responses by analyzing transcript levels of the JA-responsive gene <i>OsbHLH148</i> and the GA-responsive gene <i>OsPIL14</i> in <i>OsJAZ9</i>-overexpressing (<i>OsJAZ9-Ox</i>) and <i>OsJAZ9</i> mutant plants. <i>OsbHLH148</i> and <i>OsPIL14</i> encode key transcription factors controlling JA and GA responses, respectively, and JA and GA antagonistically regulate their expression. In <i>OsJAZ9-Ox</i>, the expression of <i>OsbHLH148</i> was downregulated and the expression of <i>OsPIL14</i> was upregulated. By contrast, in <i>OsJAZ9</i> mutants, the expression of <i>OsbHLH148</i> was upregulated and the expression of <i>OsPIL14</i> was downregulated. These observations indicated that <i>OsJAZ9</i> regulates both JA and GA responses in rice, and this finding was supported by the opposite expression patterns of <i>OsDREB1s</i>, downstream targets of OsbHLH148 and OsPIL14, in the <i>OsJAZ9-Ox</i> and <i>OsJAZ9</i> plants. Together, these findings indicate that OsJAZ9 suppresses JA responses and promotes GA responses in rice, and the protein-protein interaction between OsJAZ9 and SLR1 is involved in the antagonistic interplay between JA and GA.
Journal Front Plant Sci
Country South Korea
Volume 9
Pages 1866
Year 2018
PubMed ID 30619427
PubMed Central ID 6305323
DOI 10.3389/fpls.2018.01866
URL -
Relation
Gene DREB1A DREB1B DREB1G PIL14 SLRL1 SLRL2 TIFY6A TIFY6B _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase