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Author Cui L.H., Min H.J., Byun M.Y., Oh H.G., Kim W.T.
Title OsDIRP1, a Putative RING E3 Ligase, Plays an Opposite Role in Drought and Cold Stress Responses as a Negative and Positive Factor, Respectively, in Rice (<i>Oryza sativa</i> L.).
Abstract:
As higher plants are sessile organisms, they are unable to move to more favorable places; thus, they have developed the ability to survive under potentially detrimental conditions. Ubiquitination is a crucial post-translational protein modification and participates in abiotic stress responses in higher plants. In this study, we identified and characterized OsDIRP1 (<i>Oryza sativa</i> Drought-Induced RING Protein 1), a nuclear-localized putative RING E3 ubiquitin (Ub) ligase in rice (<i>Oryza sativa</i> L.). <i>OsDIRP1</i> expression was induced by drought, high salinity, and abscisic acid (ABA) treatment, but not by low temperature (4°C) stress, suggesting that <i>OsDIRP1</i> is differentially regulated by different abiotic stresses. To investigate its possible role in abiotic stress responses, <i>OsDIRP1</i>-overexpressing transgenic rice plants (<i>Ubi:OsDIRP1-sGFP</i>) were generated, and their phenotypes were analyzed. The T4 <i>Ubi:OsDIRP1-sGFP</i> lines showed decreased tolerance to drought and salt stress as compared to wild-type rice plants. Moreover, <i>Ubi:OsDIRP1-sGFP</i> progeny were less sensitive to ABA than the wild-type during both germination and post-germination growth. In contrast, <i>Ubi:OsDIRP1-sGFP</i> plants exhibited markedly higher tolerance to prolonged cold (4°C) treatment. These results suggest that OsDIRP1 acts as a negative regulator during drought and salt stress, whereas it functions as a positive factor during the cold stress response in rice.
Journal Front Plant Sci
Country South Korea
Volume 9
Pages 1797
Year 2018
PubMed ID 30568669
PubMed Central ID 6290360
DOI 10.3389/fpls.2018.01797
URL -
Relation
Gene DIRP1 DREB1A DREB1B DREB1D GAD3 MDR7
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase