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| Reference | ||
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| Author | Chang M.X., Gu M., Xia Y.W., Dai X.L., Dai C.R., Zhang J., Wang S.C., Qu H.Y., Yamaji N., Feng Ma J., Xu G.H. | |
| Title | OsPHT1;3 Mediates Uptake, Translocation, and Remobilization of Phosphate under Extremely Low Phosphate Regimes. | |
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Abstract: Plant roots rely on inorganic orthophosphate (Pi) transporters to acquire soluble Pi from soil solutions that exists at micromolar levels in natural ecosystems. Here, we functionally characterized a rice (<i>Oryza sativa</i>) Pi transporter, Os Phosphate Transporter-1;3 (OsPHT1;3), that mediates Pi uptake, translocation, and remobilization. <i>OsPHT1</i>;<i>3</i> was directly regulated by Os Phosphate Starvation Response-2 and, in response to Pi starvation, showed enhanced expression in young leaf blades and shoot basal regions and even more so in roots and old leaf blades. OsPHT1;3 was able to complement a yeast mutant strain defective in five Pi transporters and mediate Pi influx in <i>Xenopus laevis</i> oocytes. Overexpression of <i>OsPHT1</i>;<i>3</i> led to increased Pi concentration both in roots and shoots. However, unlike that reported for other known OsPHT1 members that facilitate Pi uptake at relatively higher Pi levels, mutation of <i>OsPHT1</i>;<i>3</i> impaired Pi uptake and root-to-shoot Pi translocation only when external Pi concentration was below 5 mum Moreover, in basal nodes, the expression of <i>OsPHT1</i>;<i>3</i> was restricted to the phloem of regular vascular bundles and enlarged vascular bundles. An isotope labeling experiment with 32P showed that <i>OsPHT1</i>;<i>3</i> mutant lines were impaired in remobilization of Pi from source to sink leaves. Furthermore, overexpression and mutation of <i>OsPHT1</i>;<i>3</i> led to reciprocal alteration in the expression of <i>OsPHT1</i>;<i>2</i> and several other <i>OsPHT1</i> genes. Yeast-two-hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays all demonstrated a physical interaction between OsPHT1;3 and OsPHT1;2. Taken together, our results indicate that OsPHT1;3 acts as a crucial factor for Pi acquisition, root-to-shoot Pi translocation, and redistribution of phosphorus in plants growing in environments with extremely low Pi levels. |
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| Journal | Plant Physiol. | |
| Country | China | |
| Volume | 179(2) | |
| Pages | 656-670 | |
| Year | 2019 | |
| PubMed ID | 30567970 | |
| PubMed Central ID | 6426419 | |
| DOI | 10.1104/pp.18.01097 | |
| URL | - | |
| Relation | ||
| Gene | PHR2 PT2 PT3 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
