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著者 Ni L., Fu X., Zhang H., Li X., Cai X., Zhang P., Liu L., Wang Q., Sun M., Wang Q.W., Zhang A., Zhang Z., Jiang M.
タイトル Abscisic Acid Inhibits Rice Protein Phosphatase PP45 via H2O2 and Relieves Repression of the Ca2+/CaM-Dependent Protein Kinase DMI3.
Abstract:
In plants, Ca2+/calmodulin-dependent protein kinase (CCaMK) is a positive regulator of abscisic acid (ABA) responses, including root growth, antioxidant defense, and tolerance of both water stress and oxidative stress. However, the underlying molecular mechanisms are poorly understood. Here, we show a direct interaction between DMI3 (Doesn't Make Infections 3), a rice (<i>Oryza sativa</i>) CCaMK and PP45, a type 2C protein phosphatase in rice (PP2C). This interaction involves the CaM binding domain of DMI3 and the PP2C domain of PP45. In the absence of ABA, PP45 directly inactivates DMI3 by dephosphorylating Thr-263 in DMI3. However, in the presence of ABA, ABA-induced H2O2 production by the NADPH oxidases RbohB/E inhibits the activity of PP45 not only by inhibiting the expression of <i>PP45</i> but also by oxidizing Cys-350 and Cys-428 residues to form PP45 intermolecular dimers. ABA-induced oxidation of Cys-350 and Cys-428 in PP45 blocked the interaction between PP45 and DMI3 and substantially prevented PP45-mediated inhibition in DMI3 activity. Genetic analysis indicated that PP45 is an important negative regulator of ABA signaling. These results reveal important pathways for the inhibition of DMI3 under the basal state and for its ABA-induced activation in rice.
掲載誌 Plant Cell
Country China
刊数 31(1)
ページ 128-152
発刊年 2019
PubMed ID 30538152
PubMed Central ID 6391686
DOI 10.1105/tpc.18.00506
URL -
関連情報
遺伝子 BIPP2C1 DMI3 DSG1 RBOHB RBOHE
INSD -
系統 野生イネ
コアコレクション 		-
突然変異系統(遺伝研 Collection) -
種子不稔系統 -
致死変異体系統 -
組織別発生段階
- 突然変異系統(遺伝子) 		-
栽培イネ品種(遺伝研 Collection) -
組織別発生段階 -
/rice/oryzabase