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Reference Detail
| Reference | ||
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| Author | Matsuta S., Nishiyama A., Chaya G., Itoh T., Miura K., Iwasaki Y. | |
| Title | Characterization of Heterotrimeric G Protein gamma4 Subunit in Rice. | |
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Abstract: Heterotrimeric G proteins are the molecule switch that transmits information from external signals to intracellular target proteins in mammals and yeast cells. In higher plants, heterotrimeric G proteins regulate plant architecture. Rice harbors one canonical alpha subunit gene (<i>RGA1</i>), four extra-large GTP-binding protein genes (XLGs), one canonical beta-subunit gene (<i>RGB1</i>), and five gamma-subunit genes (tentatively designated <i>RGG1</i>, <i>RGG2</i>, <i>RGG3</i>/<i>GS3</i>/<i>Mi</i>/<i>OsGGC1</i>, <i>RGG4</i>/<i>DEP1</i>/<i>DN1</i>/<i>qPE9-1</i>/<i>OsGGC3</i>, and <i>RGG5</i>/<i>OsGGC2</i>) as components of the heterotrimeric G protein complex. Among the five gamma-subunit genes, <i>RGG1</i> encodes the canonical gamma-subunit, <i>RGG2</i> encodes a plant-specific type of gamma-subunit with additional amino acid residues at the N-terminus, and the remaining three gamma-subunit genes encode atypical gamma-subunits with cysteine-rich C-termini. We characterized the <i>RGG4</i>/<i>DEP1</i>/<i>DN1</i>/<i>qPE9-1</i>/<i>OsGGC3</i> gene product Ggamma4 in the wild type (WT) and truncated protein Ggamma4∆Cys in the <i>RGG4</i>/<i>DEP1</i>/<i>DN1</i>/<i>qPE9-1</i>/<i>OsGGC3</i> mutant, <i>DN1-1</i>, as littele information regarding the native Ggamma4 and Ggamma4∆Cys proteins is currently available. Based on liquid chromatography-tandem mass spectrometry analysis, immunoprecipitated Ggamma4 candidates were confirmed as actual Ggamma4. Similar to alpha-(Galpha) and beta-subunits (Gbeta), Ggamma4 was enriched in the plasma membrane fraction and accumulated in the developing leaf sheath. As <i>RGG4</i>/<i>DEP1</i>/<i>DN1</i>/<i>qPE9-1</i>/<i>OsGGC3</i> mutants exhibited dwarfism, tissues that accumulated Ggamma4 corresponded to the abnormal tissues observed in <i>RGG4</i>/<i>DEP1</i>/<i>DN1</i>/<i>qPE9-1</i>/<i>OsGGC3</i> mutants. |
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| Journal | Int J Mol Sci | |
| Country | Japan | |
| Volume | 19(11) | |
| Pages | ||
| Year | 2018 | |
| PubMed ID | 30441812 | |
| PubMed Central ID | 6274817 | |
| DOI | 10.3390/ijms19113596 | |
| URL | - | |
| Relation | ||
| Gene | DEP1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
