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Author Huang Y., Bai X., Luo M., Xing Y.
Title short panicle 3 controls panicle architecture by upregulating APO2/RFL and increasing cytokinin content in rice.
Abstract:
Inflorescence architecture is a major determinant of spikelet numbers per panicle, a key component of grain yield in rice. In this study, short panicle 3 (sp3) was identified from a short panicle 3 (sp3) mutant in which T-DNA was inserted in the promoter of sp3, resulting in a knockdown mutation. sp3 encodes a DNA binding with one finger (Dof) transcriptional activator. Quantitative real time (qRT)-PCR and RNA in situ hybridization assays confirmed that sp3 is preferentially expressed in the young rice inflorescence, specifically in the branch primordial regions. sp3 acts as a negative regulator of inflorescence meristem abortion by upregulating APO2/RFL. sp3 both up- and downregulates expression of genes involved in cytokinin biosynthesis and catabolism, respectively. Consequently, cytokinin concentrations are decreased in young sp3 panicles, thereby leading to small panicles having fewer branches and spikelets. Our findings support a model in which sp3 regulates panicle architecture by modulating cytokinin homeostasis. Potential applications to rice breeding, through gene-editing of the sp3 promoter are assessed.
Journal J Integr Plant Biol
Country China
Volume
Pages
Year 2018
PubMed ID 30302902
PubMed Central ID -
DOI 10.1111/jipb.12729
URL -
Relation
Gene CKX3 CKX5 CKX9 GN1A IPT3 IPT7 RFL
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
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