Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Phule A.S., Barbadikar K.M., Madhav M.S., Senguttuvel P., Babu MBBP., Ananda Kumar P. | |
| Title | Genes encoding membrane proteins showed stable expression in rice under aerobic condition: novel set of reference genes for expression studies. | |
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Abstract: A set of reference genes expressing stably under aerobic and anaerobic conditions in rice is essential to execute omics studies relating to aerobic adaptations. Stability of expression of ten rice reference genes, viz. <i>Actin, eEF-1a, eIF-5C, Exp1, Exp2, Memp, SKP1A, TF-SUI1, TPH</i>, and <i>UBQ5</i> was validated across six experimental sets in shoot and root tissues at seedling, tillering, and panicle initiation stages. Comprehensively, <i>Memp</i> (Membrane protein), <i>TPH</i> (Tumor protein homolog), and <i>Exp1</i> (Expressed protein) were revealed as the most stable set with acceptable <i>M</i> and <i>V</i> value according to the gold standards of qRT-PCR using various algorithms/tools. The identified set of reference genes was validated using root trait genes, which showed concurrence with the functional expression patterns in the aerobic and anaerobic adapted cultivars. The <i>Memp</i> (Membrane protein), <i>TPH</i> (Tumor protein homolog), and <i>Exp1</i> (Expressed protein) genes are the most stable reference genes across the root and shoot at various developmental stages under aerobic and anaerobic conditions in rice. This is the first study for accurate and reliable relative gene expression analysis in rice grown in aerobic and anaerobic conditions. |
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| Journal | 3 Biotech | |
| Country | unknown | |
| Volume | 8(9) | |
| Pages | 383 | |
| Year | 2018 | |
| PubMed ID | 30148033 | |
| PubMed Central ID | 6107477 | |
| DOI | 10.1007/s13205-018-1406-9 | |
| URL | - | |
| Relation | ||
| Gene | AMT1;1 AMT1;2 AMT2;1 AMT2;3 AMT3;1 AMT3;3 ARF8 BHLH133 CML31 CRL4 DRO1 EXPB5 HKT7 HTNE IAA13 MRS2-2 NRR ONAC2 PTR2 RR2 SPX1 WRKY55 YUCCA1 _ _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
