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Reference Detail
| Reference | ||
|---|---|---|
| Author | Sun Y., Luo W., Jain A., Liu L., Ai H., Liu X., Feng B., Zhang L., Zhang Z., Guohua X., Sun S. | |
| Title | OsPHR3 affects the traits governing nitrogen homeostasis in rice. | |
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Abstract: Phosphate (Pi) and Nitrogen (N) are essential macronutrients required for plant growth and development. In Arabidopsis thaliana (Arabidopsis), the transcription factor PHR1 acts as a Pi central regulator. PHL1 is a homolog of PHR1 and also plays a role in maintaining Pi homeostasis. In rice (Oryza sativa), OsPHR1-4 are the orthologs of PHR1 and have been implicated in regulating sensing and signaling cascades governing Pi homeostasis. Here the role of OsPHR3 was examined in regulating the homeostasis of N under different Pi regimes. Deficiencies of different variants of N exerted attenuating effects on the relative expression levels of OsPHR3 in a tissue-specific manner. For the functional characterization of OsPHR3, its Tos17 insertion homozygous mutants i.e., OsPHR3-1, OsPHR3-2, and OsPHR3-3 were compared with the wild-type for various morphophysiological and molecular traits during vegetative (hydroponics with different regimes of N variants) and reproductive (pot soil) growth phases. During vegetative growth phase, compared with the wild-type, OsPHR3 mutants showed significant variations in the adventitious root development, influx rates of 15N-NO3- and 15N-NH4+, concentrations of total N, NO3- and NH4+ in different tissues, and the relative expression levels of OsNRT1.1a, OsNRT2.4, OsAMT1;1, OsNia1 and OsNia2. The effects of the mutation in OsPHR3 was also explicit on the seed-set and grain yield during growth in a pot soil. Although Pi deficiency affected total N and NO3- concentration, the lateral root development and the relative expression levels of some of the NO3- and NH4+ transporter genes, its availability did not exert any notable regulatory influences on the traits governing N homeostasis. OsPHR3 plays a pivotal role in regulating the homeostasis of N independent of Pi availability. |
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| Journal | BMC Plant Biol. | |
| Country | China | |
| Volume | 18(1) | |
| Pages | 241 | |
| Year | 2018 | |
| PubMed ID | 30332988 | |
| PubMed Central ID | 6192161 | |
| DOI | 10.1186/s12870-018-1462-7 | |
| URL | - | |
| Relation | ||
| Gene | AMT1;1 AMT1;2 AMT1;3 NRT1 NRT2.4 PHR3 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
