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Reference Detail
| Reference | ||
|---|---|---|
| Author | Ma W., Zou L., Zhiyuan J.I., Xiameng X.U., Zhengyin X.U., Yang Y., Alfano J.R., Chen G. | |
| Title | Xanthomonas oryzae pv. oryzae TALE proteins recruit OsTFIIAgamma1 to compensate for the absence of OsTFIIAgamma5 in bacterial blight in rice. | |
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Abstract: Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight (BB) of rice, uses transcription activator-like effectors (TALEs) to interact with the basal transcription factor gamma subunit OsTFIIAgamma5 (xa5) and activates the transcription of host genes. However, how OsTFIIAgamma1, the other OsTFIIAgamma protein, functions in the presence of TALEs remains unclear. In this study, we show that OsTFIIAgamma1 plays a compensatory role in the absence of xa5. The expression of OsTFIIAgamma1, which is activated by TALE PthXo7, increases the expression of host genes targeted by avirulent and virulent TALEs. Defective OsTFIIAgamma1 rice lines show reduced expression of the TALE-targeted susceptibility (S) genes, OsSWEET11 and OsSWEET14, which results in increased BB resistance. Selected TALEs (PthXo1, AvrXa7 and AvrXa27) were evaluated for interactions with OsTFIIAgamma1, xa5 and xa5 (naturally occurring mutant form of xa5) using biomolecular fluorescence complementation (BiFC) and microscale thermophoresis (MST). BiFC and MST demonstrated that the three TALEs bind xa5 and OsTFIIAgamma1 with a stronger affinity than xa5. These results provide insights into the complex roles of OsTFIIAgamma1 and OsTFIIAgamma5 in TALE-mediated host gene transcription. |
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| Journal | Mol. Plant Pathol. | |
| Country | China | |
| Volume | 19(10) | |
| Pages | 2248-2262 | |
| Year | 2018 | |
| PubMed ID | 29704467 | |
| PubMed Central ID | - | |
| DOI | 10.1111/mpp.12696 | |
| URL | - | |
| Relation | ||
| Gene | TFIIAGAMMA1 XA13 XA41 XA5 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
