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Reference Detail
| Reference | ||
|---|---|---|
| Author | Hu Q., Zhang C., Xue Z., Ma L., Liu W., Shen Y., Ma B., Cheng Z. | |
| Title | Osrad17 Is Required for Meiotic Double-Strand Break Repair and Plays a Redundant Role With OsZIP4 in Synaptonemal Complex Assembly. | |
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Abstract: The repair of SPO11-dependent double-strand breaks (DSBs) by homologous recombination (HR) ensures the correct segregation of homologous chromosomes. In yeast and human, RAD17 is involved in DNA damage checkpoint control and DSB repair. However, little is known about its function in plants. In this study, we characterized the RAD17 homolog in rice. In <i>Osrad17</i> pollen mother cells (PMCs), associations between non-homologous chromosomes and chromosome fragmentation were constantly observed. These aberrant chromosome associations were dependent on the formation of programmed DSBs. Osrad17 interacts with Osrad1 and the meiotic phenotype of <i>Osrad1 Osrad17</i> is indistinguishable from the two single mutants which have similar phenotypes, manifesting they could act in the same pathway. OsZIP4, OsMSH5 and OsMER3 are members of ZMM proteins in rice that are required for crossover formation. We found that homologous pairing and synapsis, which was roughly unaffected in <i>OsZIP4</i> and <i>Osrad17</i> single mutant, was severely disturbed in the <i>OsZIP4 Osrad17</i> double mutant. Similar phenotypes were observed in the <i>OsMSH5 Osrad17</i> and <i>OsMER3 Osrad1</i> double mutants, suggesting the cooperation between the checkpoint proteins and ZMM proteins in assuring accurate HR in rice. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 9 | |
| Pages | 1236 | |
| Year | 2018 | |
| PubMed ID | 30210516 | |
| PubMed Central ID | 6123563 | |
| DOI | 10.3389/fpls.2018.01236 | |
| URL | - | |
| Relation | ||
| Gene | COM1 MSH5 RAD1 RAD17 RMC1 TOP6A1 ZIP4 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
