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Reference Detail
| Reference | ||
|---|---|---|
| Author | Qian D., Chen G., Tian L., Qu L.Q. | |
| Title | OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress. | |
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Abstract: Endoplasmic reticulum-associated protein degradation (ERAD) plays an important role in endoplasmic reticulum (ER) quality control. To date, little is known about the retrotranslocation machinery in the plant ERAD pathway. We obtained a DERLIN-like protein (OsDER1) through a SWATH-based quantitative proteomic analysis of ER membrane proteins extracted from ER-stressed rice (<i>Oryza sativa</i>) seeds. OsDER1, a homolog of yeast and mammal DER1, is localized in the ER and accumulates significantly under ER stress. Overexpression or suppression of OsDER1 in rice leads to activation of the unfolded protein response and hypersensitivity to ER stress, and suppression results in floury, shrunken seeds. In addition, the expression levels of polyubiquitinated proteins increased markedly in <i>OsDER1</i> overexpression or suppression transgenic rice. Coimmunoprecipitation experiments demonstrated that OsDER1 interacted with OsHRD1, OsHRD3, and OsCDC48, the essential components of the canonical ERAD pathway. Furthermore, OsDER1 associated with the signal peptide peptidase, a homolog of a component of the alternative ERAD pathway identified recently in yeast and mammals. Our data suggest that OsDER1 is linked to the ERAD pathway. |
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| Journal | Plant Physiol. | |
| Country | China | |
| Volume | 178(1) | |
| Pages | 402-412 | |
| Year | 2018 | |
| PubMed ID | 30026288 | |
| PubMed Central ID | 6130045 | |
| DOI | 10.1104/pp.18.00375 | |
| URL | - | |
| Relation | ||
| Gene | BIP1 CDC48E CRT2 DER1 HRD3 HSP17.3 HSP18.0 HSP26 HSP70-12 HSP70CP1 HSP71.1 MAL MED37_8 PDIL2-1 PDIL5-2 PSD128 SAR1A SAR1B SAR1C SPP1 SPP2 _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
