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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zeng L., Liu X., Zhou Z., Li D., Zhao X., Zhu L., Luo Y., Hu S. | |
| Title | Identification of a G2-like transcription factor, OsPHL3, functions as a negative regulator of flowering in rice by co-expression and reverse genetic analysis. | |
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Abstract: flowering time is a key trait for regional adaption and seed production in rice (Oryza sativa L.). Forward and reverse genetic studies have characterized a number of flowering-time genes. However, co-expression analysis has not been used to identify the flowering-time genes. We predicted a G2-like family transcription factor, OsPHL3, by co-expression networks analysis with photoperiodic flowering pathway genes. OsPHL3 contains a MYB-CC domain, and was localized in the nucleus with transcriptional activation potential. OsPHL3 was mainly expressed in the leaves and exhibited a circadian rhythmic expression pattern. Rice lines overexpressing OsPHL3 showed a delayed flowering time in the genetic background of TP309 under both long-day (Beijing) and short-day (Hainan) conditions. By contrast, the knockout rice lines of OsPHL3 by CRISPR/Cas9 technology promoted flowering time regardless of genetic backgrounds (i.e. Nipponbare and TP309) or day length. Further analysis indicated that OsPHL3 delayed flowering time by down-regulating the expression of Hd3a and RFT1 through promoting Hd1 under long-day conditions (LDs), or suppressing Ehd1/Hd1 under short-day conditions (SDs). Our results suggested that co-expression analysis is a useful strategy for identifying novel flowering-time genes in rice. |
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| Journal | BMC Plant Biol. | |
| Country | China | |
| Volume | 18(1) | |
| Pages | 157 | |
| Year | 2018 | |
| PubMed ID | 30081823 | |
| PubMed Central ID | 6091178 | |
| DOI | 10.1186/s12870-018-1382-6 | |
| URL | - | |
| Relation | ||
| Gene | EF1 HD3A PHR10 RFT1 SE1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
