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Author Qin R., Zeng D., Yang C., Akhter D., Alamin M., Jin X., Shi C.
Title <i>LTBSG1</i>, a New Allele of <i>BRD2</i>, Regulates Panicle and Grain Development in Rice by Brassinosteroid Biosynthetic Pathway.
Abstract:
Panicle architecture and grain size are two important agronomic traits which determine grain yield directly in rice. In the present study, a mutant named <i>ltbsg1</i> (<i>longer top branch and shorter grain 1</i>) was isolated from the cultivar &ldquo;Zhenong 34&rdquo; (<i>Oryza sativa</i> L. ssp. <i>indica</i>) by ethyl methane sulfonate (EMS) mutagenesis. The target gene was studied through phenotype observation, genetic analysis, map-based cloning and functional analysis. The histocytological analysis indicated that the elongated top branch and shorter grain of mutant <i>ltbsg1</i> were caused from the defects of cell elongation. The <i>ltbsg1</i> gene in mutant revealed a single nucleotide substitution (G-A) in the exon 2 of <i>LOC_Os10g25780</i>, causing an amino acid variation (Glycine-Arginine) in the FAD (Flavin-adenine dinucleotide)-binding domain of delta (24)-sterol reductase, which was involved in the brassinosteroid (BR) biosynthesis. <i>LTBSG1</i> was constitutively expressed and the protein was widely localized in chloroplast, nucleus and cytomembrane. The <i>ltbsg1</i> seedlings had a lower endogenous BR level and could be restored to the phenotype of wild type by exogenous BR. The <i>LTBSG1</i> knock-out lines showed similar phenotype defects as mutant <i>ltbsg1</i>, which confirmed that <i>LTBSG1</i> was responsible for top branch elongation and grain size reduction. Furthermore, <i>LTBSG1</i> along with other BR-related genes were feedback-regulated due to their obvious altered expression in mutant <i>ltbsg1</i>. This study demonstrated that <i>LTBSG1</i> could play a new role in regulating panicle and grain development by BR biosynthetic pathway.
Journal Genes (Basel)
Country China
Volume 9(6)
Pages
Year 2018
PubMed ID 29891831
PubMed Central ID 6027417
DOI 10.3390/genes9060292
URL -
Relation
Gene BLE2 BRD1 BRD2 BU1 CPD1 D11 D2 D61 DEP1 DLT EP3 FZP G1L5 GL3.1 GS5 GW2 GW8 LAX2 MADS47 RLCK173 SMG1 TGW6 _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
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