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Reference Detail
| Reference | ||
|---|---|---|
| Author | Qin R., Zeng D., Yang C., Akhter D., Alamin M., Jin X., Shi C. | |
| Title | <i>LTBSG1</i>, a New Allele of <i>BRD2</i>, Regulates Panicle and Grain Development in Rice by Brassinosteroid Biosynthetic Pathway. | |
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Abstract: Panicle architecture and grain size are two important agronomic traits which determine grain yield directly in rice. In the present study, a mutant named <i>ltbsg1</i> (<i>longer top branch and shorter grain 1</i>) was isolated from the cultivar “Zhenong 34” (<i>Oryza sativa</i> L. ssp. <i>indica</i>) by ethyl methane sulfonate (EMS) mutagenesis. The target gene was studied through phenotype observation, genetic analysis, map-based cloning and functional analysis. The histocytological analysis indicated that the elongated top branch and shorter grain of mutant <i>ltbsg1</i> were caused from the defects of cell elongation. The <i>ltbsg1</i> gene in mutant revealed a single nucleotide substitution (G-A) in the exon 2 of <i>LOC_Os10g25780</i>, causing an amino acid variation (Glycine-Arginine) in the FAD (Flavin-adenine dinucleotide)-binding domain of delta (24)-sterol reductase, which was involved in the brassinosteroid (BR) biosynthesis. <i>LTBSG1</i> was constitutively expressed and the protein was widely localized in chloroplast, nucleus and cytomembrane. The <i>ltbsg1</i> seedlings had a lower endogenous BR level and could be restored to the phenotype of wild type by exogenous BR. The <i>LTBSG1</i> knock-out lines showed similar phenotype defects as mutant <i>ltbsg1</i>, which confirmed that <i>LTBSG1</i> was responsible for top branch elongation and grain size reduction. Furthermore, <i>LTBSG1</i> along with other BR-related genes were feedback-regulated due to their obvious altered expression in mutant <i>ltbsg1</i>. This study demonstrated that <i>LTBSG1</i> could play a new role in regulating panicle and grain development by BR biosynthetic pathway. |
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| Journal | Genes (Basel) | |
| Country | China | |
| Volume | 9(6) | |
| Pages | ||
| Year | 2018 | |
| PubMed ID | 29891831 | |
| PubMed Central ID | 6027417 | |
| DOI | 10.3390/genes9060292 | |
| URL | - | |
| Relation | ||
| Gene | BIN2 BLE2 BRD1 BRD2 BU1 CPD1 D11 D2 D61 DEP1 DLT EP3 FZP G1L5 GL3.1 GS5 GW2 GW8 LAX2 MADS47 RLCK173 SMG1 TGW6 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
