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Reference Detail
| Reference | ||
|---|---|---|
| Author | Huang W., Bai G., Wang J., Zhu W., Zeng Q., Lu K., Sun S., Fang Z. | |
| Title | Two Splicing Variants of <i>OsNPF7.7</i> Regulate shoot branching and Nitrogen Utilization Efficiency in Rice. | |
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Abstract: Rice includes 93 nitrate and peptide transporters family (NPF) members that facilitate the soil uptake and internal reallocation of nitrogen for growth and development. This study demonstrated that <i>OsNPF7.7</i> had two splicing variants, and altered expression of each variant could regulate shoot branching and nitrogen utilization efficiency (NUtE) in rice. The expression of both variants was down-regulated in the buds by increased nitrogen level in the <i>Japonica</i> rice variety ZH11. The expression level of long-variant <i>OsNPF7.7-1</i> was higher in panicles at reproductive stage, however, the expression level of short-variant <i>OsNPF7.7-2</i> was higher in buds and leaves at vegetative stage compared to each other in ZH11. OsNPF7.7-1 was localized in the plasma membrane, whereas OsNPF7.7-2 was localized in the vacuole membrane. Furthermore, the results indicated that the expression level of each variant for <i>OsNPF7.7</i> determined axillary bud outgrowth, and then influenced the rice tiller number. Overexpression of <i>OsNPF7.7-1</i> could promote nitrate influx and concentration in root, whereas overexpression of <i>OsNPF7.7-2</i> could improve ammonium influx and concentration in root. RNAi and <i>OsNPF7.7</i> lines of <i>OsNPF7.7</i> showed an increased amount of amino acids in leaf sheaths, but a decreased amount in leaf blades, which affected nitrogen allocation and plant growth. The elevated expression of each variant for <i>OsNPF7.7</i> in ZH11 enhanced NUtE using certain fertilization regimes under paddy field conditions. Moreover, overexpression of each variant for <i>OsNPF7.7</i> in KY131 increased significantly the filled grain number per plant. Thus, increased each variant of <i>OsNPF7.7</i> has the potential to improve grain yield and NUtE in rice. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 9 | |
| Pages | 300 | |
| Year | 2018 | |
| PubMed ID | 29568307 | |
| PubMed Central ID | 5852072 | |
| DOI | 10.3389/fpls.2018.00300 | |
| URL | - | |
| Relation | ||
| Gene | AMT1;3 D3 FC1 GLN1;2 NPF6.5 _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
