Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Santos FICD., Marini N., Santos R.S.D., Hoffman B.S.F., Alves-Ferreira M., de Oliveira AC. | |
| Title | Selection and testing of reference genes for accurate RT-qPCR in rice seedlings under iron toxicity. | |
|
Abstract: Reverse Transcription quantitative PCR (RT-qPCR) is a technique for gene expression profiling with high sensibility and reproducibility. However, to obtain accurate results, it depends on data normalization by using endogenous reference genes whose expression is constitutive or invariable. Although the technique is widely used in plant stress analyzes, the stability of reference genes for iron toxicity in rice (Oryza sativa L.) has not been thoroughly investigated. Here, we tested a set of candidate reference genes for use in rice under this stressful condition. The test was performed using four distinct methods: NormFinder, BestKeeper, geNorm and the comparative DeltaCt. To achieve reproducible and reliable results, Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines were followed. Valid reference genes were found for shoot (P2, OsGAPDH and OsNABP), root (OsEF-1a, P8 and OsGAPDH) and root+shoot (OsNABP, OsGAPDH and P8) enabling us to perform further reliable studies for iron toxicity in both indica and japonica subspecies. The importance of the study of other than the traditional endogenous genes for use as normalizers is also shown here. |
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| Journal | PLoS ONE | |
| Country | Brazil | |
| Volume | 13(3) | |
| Pages | e0193418 | |
| Year | 2018 | |
| PubMed ID | 29494624 | |
| PubMed Central ID | 5832244 | |
| DOI | 10.1371/journal.pone.0193418 | |
| URL | - | |
| Relation | ||
| Gene | C3H3 GAPC2 RFA4 _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
