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| 文献 | ||
|---|---|---|
| 著者 | Du L., Xu F., Fang J., Gao S., Tang J., Fang S., Wang H., Tong H., Zhang F., Chu J., Wang G., Chu C. | |
| タイトル | Endosperm sugar accumulation caused by mutation of phs8/ISA1 leads to pre-harvest sprouting in rice. | |
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Abstract: Pre-harvest sprouting (PHS) is an unfavorable trait in cereal crops that could seriously decrease grain yield and quality. Although some PHS-associated quantitative trait loci or genes in cereals have been reported, the molecular mechanism underlying PHS remains largely elusive. Here, we characterized a rice mutant, phs8, which exhibits PHS phenotype accompanied by sugary endosperm. Map-based cloning revealed that phs8 encodes a starch debranching enzyme named isoamylase1. Mutation in phs8 resulted in the phytoglycogen breakdown and sugar accumulation in the endosperm. Intriguingly, with increase of sugar contents, decreased expression of OsABI3 and OsABI5 as well as reduced sensitivity to abscisic acid (ABA) were found in the phs8 mutant. Using rice suspension cell system, we confirmed that exogenous sugar is sufficient to suppress the expression of both OsABI3 and OsABI5. Furthermore, overexpression of OsABI3 or OsABI5 could partially rescue the PHS phenotype of phs8. Therefore, our study presents important evidence supporting that endosperm sugar not only acts as an essential energy source for seed germination but also determines seed dormancy and germination by affecting ABA signaling. |
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| 掲載誌 | Plant J. | |
| Country | China | |
| 刊数 | 95(3) | |
| ページ | 545-556 | |
| 発刊年 | 2018 | |
| PubMed ID | 29775500 | |
| PubMed Central ID | - | |
| DOI | 10.1111/tpj.13970 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | MFT2 PHS8 VP1 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
