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Reference Detail
| Reference | ||
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| Author | Guo S., Dai S., Singh P.K., Wang H., Wang Y., Tan J.L.H., Wee W., Ito T. | |
| Title | A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in <i>Oryza sativa</i>. | |
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Abstract: In spite of short-day (SD) nature, rice (<i>Oryza sativa</i>) shares a conserved photoperiodic network for flowering control with long-day plants like <i>Arabidopsis thaliana.</i> Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the <i>Ubiquitin</i> promoter to produce the overexpressing lines <i>OsNTL5∆C, OsNTL5</i>∆C-<i>SRDX</i>, and <i>OsNTL5∆C-VP</i>, respectively in rice. The <i>OsNTL5</i>∆<i>C-VP</i> line showed an early-flowering phenotype. In contrast to this, the plants with <i>OsNTL5</i>∆C and <i>OsNTL5</i>∆C-<i>SRDX</i> showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as <i>Early Heading Date 1</i> (<i>Ehd1</i>), <i>Heading Date 3a</i> (<i>Hd3a</i>), and <i>Rice Flowering Locus T1</i> (<i>RFT1</i>) was significantly changed in the overexpression lines of <i>OsNTL5</i>∆<i>C-VP, OsNTL5</i>∆<i>C</i>, and <i>OsNTL5</i>∆<i>C-SRDX</i> as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of <i>Ehd1</i> and negatively regulates the expression of <i>Ehd1</i>, which shows antagonistic photoperiodic expression patterns of <i>OsNTL5</i> in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of <i>Ehd1</i>. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 9 | |
| Pages | 555 | |
| Year | 2018 | |
| PubMed ID | 29774039 | |
| PubMed Central ID | 5943572 | |
| DOI | 10.3389/fpls.2018.00555 | |
| URL | - | |
| Relation | ||
| Gene | EF1 HD3A NAC40 RFT1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
