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| 著者 | Xiao H., Xu Y., Ni C., Zhang Q., Zhong F., Huang J., Liu W., Peng L., Zhu Y., Hu J. | |
| タイトル | A rice dual-localized pentatricopeptide repeat protein is involved in organellar RNA editing together with OsMORFs. | |
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Abstract: In flowering plants, various RNA editing events occur in the mitochondria and chloroplasts as part of post-transcriptional processes. Although several pentatricopeptide repeat (PPR) proteins and multiple organellar RNA editing factors (MORFs) have been identified as RNA editing factors, the underlying mechanism of PPRs and the cooperation among these proteins are still obscure. Here, we identified a rice dual-localized PPR protein, OsPGL1. The loss of function of OsPGL1 resulted in defects in both chloroplast RNA editing of ndhD-878 and mitochondrial RNA editing of ccmFc-543, both of which could be restored in transgenic complementation lines. Despite synonymous editing of ccmFc-543, the loss of editing of ndhD-878 caused a failed conversion of serine to leucine, leading to chloroplast dysfunction and defects in the photosynthetic complex; the results of additional experiments demonstrated that OsPGL1 directly binds to both transcripts. Interactions between three OsMORFs (OsMORF2/8/9) and OsPGL1 both in vitro and in vivo were confirmed, implying that OsPGL1 functions in RNA editing via an editosome. These findings also suggested that OsMORFs assist with and contribute to a flexible PPR-RNA recognition model during RNA editing. These results indicate that, in cooperation with PPRs, OsPGL1 is required for RNA editing. In addition, our study provides new insights into the relationship between RNA editing and plant development. |
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| 掲載誌 | J. Exp. Bot. | |
| Country | China | |
| 刊数 | 69(12) | |
| ページ | 2923-2936 | |
| 発刊年 | 2018 | |
| PubMed ID | 29562289 | |
| PubMed Central ID | 5972581 | |
| DOI | 10.1093/jxb/ery108 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | CAB1R HEMA MORF1 MORF2 MORF3 MORF8 MORF8A MORF9 NDHD PETB PGL1 PSAA PSBA RBCL RBCS RPOA RPOB RPOTP ST1 WSP1 YGL1 _ _ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
