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Reference Detail
| Reference | ||
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| Author | Sim J.S., Kesawat M.S., Kumar M., Kim S.Y., Mani V., Subramanian P., Park S., Lee C.M., Kim S.R., Hahn B.S. | |
| Title | Lack of the alpha1,3-Fucosyltransferase Gene (<i>OsFucT</i>) Affects Anther Development and pollen Viability in Rice. | |
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Abstract: <i>N</i>-linked glycosylation is one of the key post-translational modifications. α1,3-Fucosyltransferase (OsFucT) is responsible for transferring α1,3-linked fucose residues to the glycoprotein <i>N</i>-glycan in plants. We characterized an <i>OsFucT</i> mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions. In addition, the anthers were curved, the pollen grains were shriveled, and pollen viability and pollen number per anther decreased dramatically in the mutant. Matrix-assisted laser desorption/ionization time-of-flight analyses of the <i>N</i>-glycans revealed that α1,3-fucose was lacking in the <i>N</i>-glycan structure of the mutant. Mutant complementation revealed that the phenotype was caused by loss of <i>OsFucT</i> function. Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins. Moreover, the mutant exhibited sensitivity to an increased concentration of salt. This study facilitates a further understanding of the function of genes mediating <i>N</i>-glycan modification and anther and pollen development in rice. |
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| Journal | Int J Mol Sci | |
| Country | South Korea | |
| Volume | 19(4) | |
| Pages | ||
| Year | 2018 | |
| PubMed ID | 29670011 | |
| PubMed Central ID | 5979348 | |
| DOI | 10.3390/ijms19041225 | |
| URL | - | |
| Relation | ||
| Gene | BHLH6 EIF4A2 FUCTA ICL KCS12 KIF19 MRLK61 RPBF WAK101 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
