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Reference Detail
| Reference | ||
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| Author | Moin M., Bakshi A., Madhav M.S., Kirti P.B. | |
| Title | Expression Profiling of Ribosomal Protein Gene Family in Dehydration Stress Responses and Characterization of Transgenic Rice Plants Overexpressing <i>RPL23A</i> for Water-Use Efficiency and Tolerance to Drought and Salt Stresses. | |
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Abstract: Our previous findings on the screening of a large-pool of activation tagged rice plants grown under limited water conditions revealed the activation of Ribosomal Protein Large (RPL) subunit genes, <i>RPL6</i> and <i>RPL23A</i> in two mutants that exhibited high water-use efficiency (WUE) with the genes getting activated by the integrated 4x enhancers (Moin et al., 2016a). In continuation of these findings, we have comprehensively characterized the Ribosomal Protein (RP) gene family including both small (RPS) and large (RPL) subunits, which have been identified to be encoded by at least 70 representative genes; RP-genes exist as multiple expressed copies with high nucleotide and amino acid sequence similarity. The differential expression of all the representative genes in rice was performed under limited water and drought conditions at progressive time intervals in the present study. More than 50% of the RP genes were upregulated in both shoot and root tissues. Some of them exhibited an overlap in upregulation under both the treatments indicating that they might have a common role in inducing tolerance under limited water and drought conditions. Among the genes that became significantly upregulated in both the tissues and under both the treatments are <i>RPL6, 7, 23A, 24</i>, and <i>31</i> and <i>RPS4, 10</i> and <i>18a</i>. To further validate the role of RP genes in WUE and inducing tolerance to other stresses, we have raised transgenic plants overexpressing <i>RPL23A</i> in rice. The high expression lines of <i>RPL23A</i> exhibited low Delta13C, increased quantum efficiency along with suitable growth and yield parameters with respect to negative control under the conditions of limited water availability. The constitutive expression of <i>RPL23A</i> was also associated with transcriptional upregulation of many other RPL and RPS genes. The seedlings of <i>RPL23A</i> high expression lines also showed a significant increase in fresh weight, root length, proline and chlorophyll contents under simulated drought and salt stresses. Taken together, our findings provide a secure basis for the RPL gene family expression as a potential resource for exploring abiotic stress tolerant properties in rice. |
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| Journal | Front Chem | |
| Country | India | |
| Volume | 5 | |
| Pages | 97 | |
| Year | 2017 | |
| PubMed ID | 29184886 | |
| PubMed Central ID | 5694489 | |
| DOI | 10.3389/fchem.2017.00097 | |
| URL | - | |
| Relation | ||
| Gene | BIP123 BZIP23 ERF42 NAC6 ONAC2 RPL13A RPL13B RPL15 RPL17 RPL18A RPL19 RPL2 RPL21.2 RPL23A RPL26 RPL26.1 RPL28 RPL29 RPL30E RPL31 RPL34 RPL35A.3 RPL36.2 RPL37 RPL38 RPL4 RPL44 RPL5 RPL51 RPL6 RPS10A RPS15A RPS17 RPS18A RPS23A RPS24 RPS25 RPS26 RPS27 RPS28 RPS29 RPS30 RPS3AC RPS4 RPS5 RPS6A RPS7A RPS9-2 UBQ5 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
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| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
