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Author He Y., Li L., Zhang Z., Wu J.L.
Title Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.).
Abstract:
Premature leaf senescence negatively impacts the grain yield in the important monocot rice (<i>Oryza sativa</i> L.); to understand the molecular mechanism we carried out a screen for mutants with premature senescence leaves in a mutant bank generated by ethyl methane sulfonate (EMS) mutagenesis of elite <i>indica</i> rice ZhongJian100. Five premature senescence leaf (<i>psl15</i>, <i>psl50</i>, <i>psl89</i>, <i>psl117</i> and <i>psl270</i>) mutants were identified with distinct yellowish phenotypes on leaves starting from the tillering stage to final maturation. Moreover, these mutants exhibited significantly increased malonaldehyde content, decreased chlorophyll content, reduced numbers of chloroplast and grana thylakoid, altered photosynthetic ability and expression of photosynthesis-related genes. Furthermore, the expression of senescence-related indicator <i>OsI57</i> was significantly up-regulated in four mutants. Histochemical analysis indicated that cell death and reactive oxygen species (ROS) accumulation occurred in the mutants with altered activities of ROS scavenging enzymes. Both darkness and abscisic acid (ABA) treatments could induce leaf senescence and resulted in up- or down-regulation of ABA metabolism-related genes in the mutants. Genetic analysis indicated that all the premature senescence leaf mutants were controlled by single non-allelic recessive genes. The data suggested that mechanisms underlying premature leaf senescence are likely different among the mutants. The present study would facilitate us to further fine mapping, cloning and functional characterization of the corresponding genes mediating the premature leaf senescence in rice.
Journal Int J Mol Sci
Country China
Volume 19(1)
Pages
Year 2018
PubMed ID 29301377
PubMed Central ID 5796089
DOI 10.3390/ijms19010140
URL -
Relation
Gene ABA1 ABA8OX1 ABA8OX2 ABA8OX3 CAB2R FGL NCED1 NCED1 NCED3 PSBA PSBS1 PSL117 PSL15 PSL270 PSL50 PSL89 RBCL RCCR1 SGR YGL10 YGL98 _ _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase