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Author Ren L., Tang D., Zhao T., Zhang F., Liu C., Xue Z., Shi W., Du G., Shen Y., Li Y., Cheng Z.
Title OsSPL regulates meiotic fate acquisition in rice.
In angiosperms, the key step in sexual reproduction is successful acquisition of meiotic fate. However, the molecular mechanism determining meiotic fate remains largely unknown. Here, we report that OsSPOROCYTELESS (OsSPL) is critical for meiotic entry in rice (Oryza sativa). We performed a large-scale genetic screen of rice sterile mutants aimed to identify genes regulating meiotic entry and identified OsSPL using map-based cloning. We showed that meiosis-specific callose deposition, chromatin organization, and centromere-specific histone H3 loading were altered in the cells corresponding to pollen mother cells in OsSPL anthers. Global transcriptome analysis showed that the enriched differentially expressed genes in OsSPL were mainly related to redox status, meiotic process, and parietal cell development. OsSPL might form homodimers and interact with TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factor OsTCP5 via the SPL dimerization and TCP interaction domain. OsSPL also interacts with TPL (TOPLESS) corepressors, OsTPL2 and OsTPL3, via the EAR motif. Our results suggest that the OsSPL-mediated signaling pathway plays a crucial role in rice meiotic entry, which appears to be a conserved regulatory mechanism for meiotic fate acquisition in angiosperms.
Journal New Phytol.
Country China
Volume 218(2)
Pages 789-803
Year 2018
PubMed ID 29479720
PubMed Central ID -
DOI 10.1111/nph.15017
Gene ASP1 GSL5 MEL1 MEL2 PAIR2 PHS1 RAD21-4 SPL UGP2 _ _ _ _ _ _ _
Strain Wild Core Collection -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
Stages in Each Organ
- Muant Lines (Gene)
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -