研究支援ツール - 文献 - 詳細
文献詳細
文献 | ||
---|---|---|
著者 | Lavania D., Dhingra A., Grover A. | |
タイトル | Analysis of transactivation potential of rice (Oryza sativa L.) heat shock factors. | |
Abstract: Based on yeast one-hybrid assays, we show that the presence of C-terminal AHA motifs is not a prerequisite for transactivation potential in rice heat shock factors. Transcriptional activation or transactivation (TA) of heat stress responsive genes takes place by binding of heat shock factors (Hsfs) to heat shock elements. Analysis of TA potential of thirteen rice (Oryza sativa L.) Hsfs (OsHsfs) carried out in this study by yeast one-hybrid assay showed that OsHsfsA3 possesses strong TA potential while OsHsfs A1a, A2a, A2b, A4a, A4d, A5, A7b, B1, B2a, B2b, B2c and B4d lack TA potential. From a near complete picture of TA potential of the OsHsf family (comprising of 25 members) emerging from this study and an earlier report from our group (Mittal et al. in FEBS J 278(17):3076-3085, 2011), it is concluded that (1) overall, six OsHsfs, namely A3, A6a, A6b, A8, C1a and C1b possess TA potential; (2) four class A OsHsfs, namely A3, A6a, A6b and A8 have TA potential out of which A6a and A6b contain AHA motifs while A3 and A8 lack AHA motifs; (3) nine class A OsHsfs, namely A1a, A2a, A2b, A2e, A4a, A4d, A5, A7a and A7b containing AHA motif(s) lack TA function in the yeast assay system; (4) all class B OsHsfs lack AHA motifs and TA potential (B4a not analyzed) and (5) though all class C OsHsf members lack AHA motifs, two members C1a and C1b possess TA function, while one member C2a lacks TA potential (C2b not analyzed). Thus, the presence or absence of AHA motif is possibly not the only factor determining TA potential of OsHsfs. Our findings will help to identify the transcriptional activators of rice heat shock response. |
||
掲載誌 | Planta | |
Country | India | |
刊数 | 247(6) | |
ページ | 1267-1276 | |
発刊年 | 2018 | |
PubMed ID | 29453664 | |
PubMed Central ID | - | |
DOI | 10.1007/s00425-018-2865-2 | |
URL | - | |
関連情報 | ||
遺伝子 | HSFA1 HSFA2A HSFA2B HSFA2C HSFA2D HSFA2E HSFA3 HSFA4A HSFA5 HSFA6 HSFA7 HSFA9 HSFB1 HSFB2A HSFB2B HSFB2C HSFB4A HSFB4B HSFB4C HSFB4D HSFC1A HSFC1B HSFC2A HSFC2B SPL7 | |
INSD | - | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |