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| 著者 | Ning X., Sun Y., Wang C., Zhang W., Sun M., Hu H., Liu J., Yang L. | |
| タイトル | A Rice CPYC-type Glutaredoxin <i>OsGRX20</i> in Protection against Bacterial Blight, Methyl Viologen and Salt Stresses. | |
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Abstract: Glutaredoxins (GRXs) belong to the antioxidants involved in the cellular stress responses. In spite of the identification 48 <i>GRX</i> genes in rice genomes, the biological functions of most of them remain unknown. Especially, the biological roles of members of <i>GRX</i> family in disease resistance are still lacking. Our proteomic analysis found that OsGRX20 increased by 2.7-fold after infection by bacterial blight. In this study, we isolated and characterized the full-length nucleotide sequences of the rice <i>OsGRX20</i> gene, which encodes a GRX family protein with CPFC active site of CPYC-type class. OsGRX20 protein was localized in nucleus and cytosol, and its transcripts were expressed predominantly in leaves. Several stress- and hormone-related motifs putatively acting as regulatory elements were found in the <i>OsGRX20</i> promoter. Real-time quantitative PCR analysis indicated that <i>OsGRX20</i> was expressed at a significantly higher level in leaves of a resistant or tolerant rice genotype, Yongjing 50A, than in a sensitive genotype, Xiushui 11, exposed to bacterial blight, methyl viologen, heat, and cold. Its expression could be induced by salt, PEG-6000, 2,4-D, salicylic acid, jasmonic acid, and abscisic acid treatments in Yongjing 50A. Overexpression of <i>OsGRX20</i> in rice Xiushui 11 significantly enhanced its resistance to bacterial blight attack, and tolerance to methyl viologen and salt stresses. In contrast, interference of <i>OsGRX20</i> in Yongjing 50A led to increased susceptibility to bacterial blight, methyl viologen and salt stresses. <i>OsGRX20</i> restrained accumulation of superoxide radicals in aerial tissue during methyl viologen treatment. Consistently, alterations in <i>OsGRX20</i> expression affect the ascorbate/dehydroascorbate ratio and the abundance of transcripts encoding four reactive oxygen species scavenging enzymes after methyl viologen-induced stress. Our results demonstrate that OsGRX20 functioned as a positive regulator in rice tolerance to multiple stresses, which may be of significant use in the genetic improvement of rice resistance. |
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| 掲載誌 | Front Plant Sci | |
| Country | China | |
| 刊数 | 9 | |
| ページ | 111 | |
| 発刊年 | 2018 | |
| PubMed ID | 29479359 | |
| PubMed Central ID | 5811478 | |
| DOI | 10.3389/fpls.2018.00111 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | APX1 CATB GRX20 NOE1 SODB | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
