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Reference Detail
| Reference | ||
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| Author | Wang Z., Tian X., Zhao Q., Liu Z., Li X., Ren Y., Tang J., Fang J., Xu Q., Bu Q. | |
| Title | The E3 Ligase DROUGHT HYPERSENSITIVE Negatively Regulates Cuticular Wax Biosynthesis by Promoting the Degradation of Transcription Factor ROC4 in Rice. | |
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Abstract: Cuticular wax plays crucial roles in protecting plants from environmental stresses, particularly drought stress. Many enzyme-encoding genes and transcription factors involved in wax biosynthesis have been identified, but the underlying posttranslational regulatory mechanisms are poorly understood. Here, we demonstrate that <i>DROUGHT HYPERSENSITIVE</i> (<i>DHS</i>), encoding a Really Interesting New Gene (RING)-type protein, is a critical regulator of wax biosynthesis in rice (<i>Oryza sativa</i>). The cuticular wax contents were significantly reduced in <i>DHS</i> overexpression plants but increased in <i>DHS</i> mutants compared with the wild type, which resulted in a response opposite that of drought stress. DHS exhibited E3 ubiquitin ligase activity and interacted with the homeodomain-leucine zipper IV protein ROC4. Analysis of <i>ROC4</i> overexpression plants and <i>ROC4</i> mutants indicated that ROC4 positively regulates cuticular wax biosynthesis and the drought stress response. ROC4 is ubiquitinated in vivo and subjected to ubiquitin/26S proteasome-mediated degradation. ROC4 degradation was promoted by DHS but delayed in <i>DHS</i> mutants. ROC4 acts downstream of DHS, and Os<i>-BDG</i> is a direct downstream target of the DHS-ROC4 cascade. These results suggest a mechanism whereby DHS negatively regulates wax biosynthesis by promoting the degradation of ROC4, and they suggest that DHS and ROC4 are valuable targets for the engineering of drought-tolerant rice cultivars. |
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| Journal | Plant Cell | |
| Country | China | |
| Volume | 30(1) | |
| Pages | 228-244 | |
| Year | 2018 | |
| PubMed ID | 29237723 | |
| PubMed Central ID | 5810576 | |
| DOI | 10.1105/tpc.17.00823 | |
| URL | - | |
| Relation | ||
| Gene | BDG DHS ROC4 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
