更新情報研究支援ツール - 文献 - 詳細
文献詳細
| 文献 | ||
|---|---|---|
| 著者 | Wang J., Islam F., Li L., Long M., Yang C., Jin X., Ali B., Mao B., Zhou W. | |
| タイトル | Complementary RNA-Sequencing Based Transcriptomics and iTRAQ Proteomics Reveal the Mechanism of the Alleviation of Quinclorac Stress by Salicylic Acid in Oryza sativa ssp. japonica. | |
|
Abstract: To uncover the alleviation mechanism of quinclorac stress by salicylic acid (SA), leaf samples of Oryza sativa ssp. Japonica under quinclorac stress with and without SA pre-treatment were analyzed for transcriptional and proteomic profiling to determine the differentially expressed genes (DEGs) and proteins (DEPs), respectively. Results showed that quinclorac stress altered the expression of 2207 DEGs (1427 up-regulated, 780 down-regulated) and 147 DEPs (98 down-regulated, 49 up-regulated). These genes and proteins were enriched in glutathione (GSH) metabolism, porphyrin and chlorophyll metabolism, the biosynthesis of secondary metabolites, glyoxylate and dicarboxylate metabolism, and so on. It also influenced apetala2- ethylene-responsive element binding protein (AP2-EREBP) family, myeloblastosis (MYB) family and WRKY family transcription factors. After SA pre-treatment, 697 genes and 124 proteins were differentially expressed. Pathway analysis showed similar enrichments in GSH, glyoxylate and dicarboxylate metabolism. Transcription factors were distributed in basic helix-loop-helix (bHLH), MYB, Tify and WRKY families. Quantitative real-time PCR results revealed that quinclorac stress induced the expression of glutathion reductase (GR) genes (OsGR2, OsGR3), which was further pronounced by SA pre-treatment. Quinclorac stress further mediated the accumulation of acetaldehyde in rice, while SA enhanced the expression of OsALDH2B5 and OsALDH7 to accelerate the metabolism of herbicide quinclorac for the protection of rice. Correlation analysis between transcriptome and proteomics demonstrated that, under quinclorac stress, correlated proteins/genes were mainly involved in the inhibition of intermediate steps in the biosynthesis of chlorophyll. Other interesting proteins/genes and pathways regulated by herbicide quinclorac and modulated by SA pre-treatment were also discussed, based on the transcriptome and proteomics results. |
||
| 掲載誌 | Int J Mol Sci | |
| Country | China | |
| 刊数 | 18(9) | |
| ページ | ||
| 発刊年 | 2017 | |
| PubMed ID | 28906478 | |
| PubMed Central ID | 5618624 | |
| DOI | 10.3390/ijms18091975 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | 4CL4 ALDH2A ALDH7 BGLU7 DREB2A EREBP1 ERF92 GR2 GR3 IRO2 MDH12.1 NAC122 NAC95 PAL PAL1 PAL1 PHR3 PK1 WRKY45 WRKY76 _ _ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
