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| 著者 | Ko S.S., Li M.J., Lin Y.J., Hsing H.X., Yang T.T., Chen T.K., Jhong C.M., Ku M.S. | |
| タイトル | Tightly Controlled Expression of bHLH142 Is Essential for Timely Tapetal Programmed Cell Death and pollen Development in Rice. | |
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Abstract: male sterility is important for hybrid seed production. pollen development is regulated by a complex network. We previously showed that knockout of bHLH142 in rice (Oryza sativa) causes pollen sterility by interrupting tapetal programmed cell death (PCD) and bHLH142 coordinates with TDR to modulate the expression of EAT1. In this study, we demonstrated that overexpression of bHLH142 (OE142) under the control of the ubiquitin promoter also leads to male sterility in rice by triggering the premature onset of PCD. Protein of bHLH142 was found to accumulate specifically in the OE142 anthers. Overexpression of bHLH142 induced early expression of several key regulatory transcription factors in pollen development. In particular, the upregulation of EAT1 at the early stage of pollen development promoted premature PCD in the OE142 anthers, while its downregulation at the late stage impaired pollen development by suppressing genes involved in pollen wall biosynthesis, ROS scavenging and PCD. Collectively, these events led to male sterility in OE142. Analyses of related mutants further revealed the hierarchy of the pollen development regulatory gene network. Thus, the findings of this study advance our understanding of the central role played by bHLH142 in the regulatory network leading to pollen development in rice and how overexpression of its expression affects pollen development. Exploitation of this novel functionality of bHLH142 may confer a big advantage to hybrid seed production. |
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| 掲載誌 | Front Plant Sci | |
| Country | Taiwan | |
| 刊数 | 8 | |
| ページ | 1258 | |
| 発刊年 | 2017 | |
| PubMed ID | 28769961 | |
| PubMed Central ID | 5513933 | |
| DOI | 10.3389/fpls.2017.01258 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | AP37 BHLH142 C6 CP1 CYP704B2 EAT1 GAMYB MS2 MT2B OSC4 PTC1 RADC1 RAG TDF1 TDR UDT1 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
