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Reference Detail
| Reference | ||
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| Author | Brazier-Hicks M., Gershater M., Dixon D., Edwards R. | |
| Title | Substrate specificity and safener inducibility of the plant UDP-glucose-dependent family 1 glycosyltransferase super-family. | |
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Abstract: Plants contain large numbers of family 1 UDP-glucose-dependent glycosyltransferases (UGTs), including members that conjugate xenobiotics. Arabidopsis contains 107 UGT genes with 99 family members successfully expressed as glutathione transferase (GST)-fusion proteins in E. coli. A high-throughput catalytic screen was developed based on quantification of the fusion by measuring GST activity. UGT activity using UDP-glucose as donor was then determined using 11 synthetic acceptors bearing hydroxyl, amino and thiol groups that had been shown to undergo conjugation in plant extracts. In total, 44 UGTs, largely members of the D and E groups, were active towards xenobiotics, glucosylating phenol and thiol acceptors. In contrast, N-glucosyltransferase (NGT) activity was almost exclusively restricted to a single enzyme, UGT72B1. Using DNA microarrays, the induction of UGT transcripts following treatment with the herbicide safener fenclorim was compared in Arabidopsis and rice. D and L group members were the most safener-inducible UGTs in both species. The respective Arabidopsis enzymes showed low conjugating activity towards xenobiotics. Using Genevestigator, a small group of safened D and L UGTs were consistently induced in response to biotic and abiotic stress suggestive of protective activities beyond xenobiotic detoxification in both species. The induction of other detoxifying gene families following treatment with fenclorim, namely cytochromes P450 and glutathione transferases, further confirmed the selective enhancement of related subfamily members in the two species giving new insight into the safening response in cereals, where herbicide tolerance is enhanced compared with dicots, which are unresponsive to these treatments. |
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| Journal | Plant Biotechnol. J. | |
| Country | UK | |
| Volume | ||
| Pages | ||
| Year | 2017 | |
| PubMed ID | 28640934 | |
| PubMed Central ID | - | |
| DOI | 10.1111/pbi.12775 | |
| URL | - | |
| Relation | ||
| Gene | BEL CYP51H5 CYP704A5 CYP709C11 CYP709C5 CYP709C9 CYP71AA2 CYP71AA3 CYP71AK1 CYP71E5 CYP71K7 CYP71K9 CYP71X12 CYP71X14 CYP71Y5 CYP71Y7 CYP71Z2 CYP71Z7 CYP72A17 CYP72A18 CYP72A19 CYP72A32 CYP734A2 CYP76M7 CYP76M7 CYP78C6 CYP81A5 CYP87C2 CYP89B4 CYP94C2 CYP96B5 EDR1 F5HL2 GSTU1 GSTU10 GSTU12 GSTU15 GSTU18 GSTU19 GSTU24 GSTU35 GSTU39 GSTU44 GSTU47 GSTU5 GSTU50 GSTU51 GSTU52 GSTZ2 SGT UGT701A1 UGT703A1 UGT703A2 UGT703A4 UGT710C2 UGT72F1 UGT72J2 UGT74H4 UGT75E1 UGT75K1 UGT84C1 UGT85E2 UGT87C1 UGT93B UGT98B1 UGT99B3 _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
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| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
