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| 文献 | ||
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| 著者 | Liu Q., Luo L., Wang X., Shen Z., Zheng L. | |
| タイトル | Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis. | |
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Abstract: Laccases are encoded by a multigene family and widely distributed in plant genomes where they play roles oxidizing monolignols to produce higher-order lignin involved in plant development and stress responses. We identified 30 laccase genes (OsLACs) from rice, which can be divided into five subfamilies, mostly expressed during early development of the endosperm, growing roots, and stems. OsLACs can be induced by hormones, salt, drought, and heavy metals stresses. The expression level of OsLAC10 increased 1200-fold after treatment with 20 muM Cu for 12 h. The laccase activities of OsLAC10 were confirmed in an Escherichia coli expression system. Lignin accumulation increased in the roots of Arabidopsis over-expressing OsLAC10 (OsLAC10-OX) compared to wild-type controls. After growth on 1/2 Murashige and Skoog (MS) medium containing toxic levels of Cu for seven days, roots of the OsLAC10-OX lines were significantly longer than those of the wild type. Compared to control plants, the Cu concentration decreased significantly in roots of the OsLAC10-OX line under hydroponic conditions. These results provided insights into the evolutionary expansion and functional divergence of OsLAC family. In addition, OsLAC10 is likely involved in lignin biosynthesis, and reduces the uptake of Cu into roots required for Arabidopsis to develop tolerance to Cu. |
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| 掲載誌 | Int J Mol Sci | |
| Country | China | |
| 刊数 | 18(2) | |
| ページ | ||
| 発刊年 | 2017 | |
| PubMed ID | 28146098 | |
| PubMed Central ID | 5343771 | |
| DOI | 10.3390/ijms18020209 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | LAC1 LAC10 LAC11 LAC12 LAC13 LAC15 LAC16 LAC17 LAC18 LAC19 LAC2 LAC20 LAC21 LAC22 LAC23 LAC24 LAC25 LAC26 LAC27 LAC28 LAC29 LAC3 LAC30 LAC4 LAC5 LAC6 LAC7 LAC8 LAC9 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
