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Reference Detail
| Reference | ||
|---|---|---|
| Author | Jung H., Chung P.J., Park S.H., Redillas M.C., Kim Y.S., Suh J.W., Kim J.K. | |
| Title | Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance. | |
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Abstract: The AP2/ERF family is a plant-specific transcription factor family whose members have been associated with various developmental processes and stress tolerance. Here, we functionally characterized the drought-inducible OsERF48, a group Ib member of the rice ERF family with four conserved motifs, CMI-1, -2, -3 and -4. A transactivation assay in yeast revealed that the C-terminal CMI-1 motif was essential for OsERF48 transcriptional activity. When OsERF48 was overexpressed in an either a root-specific (ROX(O)(s)(ERF)(48) ) or whole-body (OX(O)(s)(ERF)(48) ) manner, transgenic plants showed a longer and denser root phenotype compared to the nontransgenic (NT) controls. When plants were grown on a 40% polyethylene glycol-infused medium under in vitro drought conditions, ROX(O)(s)(ERF)(48) plants showed a more vigorous root growth than OX(O)(s)(ERF)(48) and NT plants. In addition, the ROX(O)(s)(ERF)(48) plants exhibited higher grain yield than OX(O)(s)(ERF)(48) and NT plants under field-drought conditions. We constructed a putative OsERF48 regulatory network by cross-referencing ROX(O)(s)(ERF)(48) root-specific RNA-seq data with a co-expression network database, from which we inferred the involvement of 20 drought-related genes in OsERF48-mediated responses. These included genes annotated as being involved in stress signalling, carbohydrate metabolism, cell-wall proteins and drought responses. They included, OsCML16, a key gene in calcium signalling during abiotic stress, which was shown to be a direct target of OsERF48 by chromatin immunoprecipitation-qPCR analysis and a transient protoplast expression assay. Our results demonstrated that OsERF48 regulates OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance. |
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| Journal | Plant Biotechnol. J. | |
| Country | South Korea | |
| Volume | ||
| Pages | ||
| Year | 2017 | |
| PubMed ID | 28244201 | |
| PubMed Central ID | - | |
| DOI | 10.1111/pbi.12716 | |
| URL | - | |
| Relation | ||
| Gene | AGP3 CCD1 CML16 DHN1 DREB1C ERF48 GOLS1 RS5 WSI76 XTH9 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
