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Author Leng Y., Yang Y., Ren D., Huang L., Dai L., Wang Y., Chen L., Tu Z., Gao Y., Li X., Zhu L., Hu J., Zhang G., Gao Z., Guo L., Kong Z., Lin Y., Qian Q., Zeng D.
Title A rice pectate lyase-LIKE gene is required for plant growth and leaf senescence.
Abstract:
To better understand the molecular mechanisms behind plant growth and leaf senescence in monocot plants, we identified a mutant exhibiting dwarfism and an early-senescence leaf phenotype, termed dwarf and early-senescence leaf (DEL1). Histological analysis showed that the abnormal growth was caused by a reduction in cell number. Further investigation revealed that the decline in cell number in DEL1 was affected by the cell cycle. Physiological analysis, transmission electron microscopy, and TUNEL assays showed that leaf senescence was triggered by the accumulation of reactive oxygen species. The DEL1 gene was cloned using a map-based approach. It was shown to encode a pectate lyase (PEL) precursor that contains a PelC domain. DEL1 contains all the conserved residues of PEL and has strong similarity with plant PelC. DEL1 is expressed in all tissues but predominantly in elongating tissues. Functional analysis revealed that mutation of DEL1 decreased the total PEL enzymatic activity, increased the degree of methylesterified homogalacturonan and altered the cell wall composition and structure. In addition, transcriptome assay revealed that a set of cell wall function and senescence related gene expression was altered in DEL1 plants. Our research indicates that DEL1 is involved in both the maintenance of normal cell division and the induction of leaf senescence. These findings reveal a new molecular mechanism for plant growth and leaf senescence mediated by pectate lyase-LIKE (PLL) genes.
Journal Plant Physiol.
Country unknown
Volume
Pages
Year 2017
PubMed ID 28455404
PubMed Central ID -
DOI 10.1104/pp.16.01625
URL -
Relation
Gene AOX1A AOX1B APX1 APX2 ATP6 CATA CATB CDC2-1 CDKF;1 CYCT1;1 DEL1 EXPB2 MCM5 NAC6 PME6 SGR SODA1 SODB WRKY23 WRKY72 _ _ _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase