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Author Shi L., Wei X.J., Adedze Y.M., Sheng Z.H., Tang S.Q., Hu P.S., Wang J.L.
Title Characterization and gene cloning of the rice (Oryza sativa L.) dwarf and narrow-leaf mutant DNL3.
Abstract:
The dwarf and narrow-leaf rice (Oryza sativa L.) mutant DNL3 was isolated from the Japonica cultivar Zhonghua 11 (wild-type). DNL3 exhibited pleiotropic developmental defects. The narrow-leaf phenotype resulted from a marked reduction in the number of vascular bundles, while the dwarf stature was caused by the formation of foreshortened internodes and a reduced number of parenchyma cells. The suggestion that cell division is impaired in the mutant was consistent with the transcriptional behavior of various genes associated with cell division. The mutant was less responsive to exogenously supplied gibberellic acid than the wild-type, and profiling the transcription of genes involved in gibberellin synthesis and response revealed that a lesion in the mutant affected gibberellin signal transduction. The DNL3 phenotype was inherited as a single-dominant gene, mapping within a 19.1-kb region of chromosome 12, which was found to harbor three open reading frames. Resequencing the open reading frames revealed that the mutant carried an allele at one of the three genes that differed from the wild-type sequence by 2-bp deletions; this gene encoded a cellulose synthase-like D4 (CSLD4) protein. Therefore, OsCSLD4 is a candidate gene for DNL3. DNL3 was expressed in all of the rice organs tested at the heading stage, particularly in the leaves, roots, and culms. These results suggest that DNL3 plays important roles in rice leaf morphogenesis and vegetative development.
Journal Genet. Mol. Res.
Country China
Volume 15(3)
Pages
Year 2016
PubMed ID 27706742
PubMed Central ID -
DOI 10.4238/gmr.15038731
URL -
Relation
Gene BC7 CESA11 CESA2 CESA3 CESA5 CESA7 CSLD5 CSLF6 GW8 MCM3 ND1 _ _ _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
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