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Reference Detail
| Reference | ||
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| Author | Fei Q., Yang L., Liang W., Zhang D., Meyers B.C. | |
| Title | Dynamic changes of small RNAs in rice spikelet development reveal specialized reproductive phasiRNA pathways. | |
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Abstract: Dissection of the genetic pathways and mechanisms by which anther development occurs in grasses is crucial for both a basic understanding of plant development and for examining traits of agronomic importance such as male sterility. In rice, MULTIPLE SPOROCYTES1 (MSP1), a leucine-rich-repeat receptor kinase, plays an important role in anther development by limiting the number of sporocytes. OsTDL1a (a TPD1-like gene in rice) encodes a small protein that acts as a cofactor of MSP1 in the same regulatory pathway. In this study, we analyzed small RNA and mRNA changes in different stages of spikelets from wild-type rice, and from MSP1 and OsTDL1a mutants. Analysis of the small RNA data identified miRNAs demonstrating differential abundances. miR2275 was depleted in the two rice mutants; this miRNA is specifically enriched in anthers and functions to trigger the production of 24-nt phased secondary siRNAs (phasiRNAs) from PHAS loci. We observed that the 24-nt phasiRNAs as well as their precursor PHAS mRNAs were also depleted in the two mutants. An analysis of co-expression identified three Argonaute-encoding genes (OsAGO1d, OsAGO2b, and OsAGO18) that accumulate transcripts coordinately with phasiRNAs, suggesting a functional relationship. By mRNA in situ analysis, we demonstrated a strong correlation between the spatiotemporal pattern of these OsAGO transcripts and phasiRNA accumulations. |
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| Journal | J. Exp. Bot. | |
| Country | USA | |
| Volume | 67(21) | |
| Pages | 6037-6049 | |
| Year | 2016 | |
| PubMed ID | 27702997 | |
| PubMed Central ID | 5100018 | |
| DOI | 10.1093/jxb/erw361 | |
| URL | - | |
| Relation | ||
| Gene | AGO18 AGO1D MEL1 MIL2 MSP1 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
