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| 著者 | He X., Li L., Xu H., Xi J., Cao X., Xu H., Rong S., Dong Y., Wang C., Chen R., Xu J., Gao X., Xu Z. | |
| タイトル | A rice jacalin-related mannose-binding lectin gene, OsJRL, enhances Escherichia coli viability under high salinity stress and improves salinity tolerance of rice. | |
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Abstract: Salinity, which is one of the most common abiotic stresses, may severely affect plant productivity and quality. Although plant lectins are thought to play important roles in plant defense signaling during pathogen attack, little is known about the contribution of plant lectins to stress resistance. We cloned and functionally characterized a rice jacalin-related mannose-binding lectin gene, OsJRL, from rice 'Nipponbare'. We analyzed the expression patterns of OsJRL under various stress conditions in rice. Furthermore, we overexpressed OsJRL in Escherichia coli and rice. The cDNA of OsJRL contained a 438 bp open reading frame, which encodes a polypeptide of 145 amino acids. OsJRL was localized in the nucleus and cytoplasm. Real time PCR analyses revealed that OsJRL expression showed tissue specificity in rice and was upregulated under diverse stresses, namely salt, drought, cold, heat and abscisic acid treatments. Overexpression of OsJRL in E. coli enhanced cell viability and dramatically improved tolerance of high salinity. Overexpression of OsJRL in rice also enhanced salinity tolerance and increased the expression levels of a number of stress-related genes, including three LEA (late embryogenesis abundant proteins) genes (OsLEA19a, OsLEA23 and OsLEA24), three Na(+) transporter genes (OsHKT1;3, OsHKT1;4 and OsHKT1;5) and two DREB genes (OsDREB1A and OsDREB2B). Based on these results, we suggest that OsJRL plays an important role in cell protection and stress signal transduction. |
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| 掲載誌 | Plant Biol (Stuttg) | |
| Country | China | |
| 刊数 | ||
| ページ | ||
| 発刊年 | 2016 | |
| PubMed ID | 27718311 | |
| PubMed Central ID | - | |
| DOI | 10.1111/plb.12514 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | DHN1 DREB1A ERF42 HKT6 HKT7 LEA3-1 LIP5 SALT SKC1 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
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| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
