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Author Wang J., Wu F., Zhu S., Xu Y., Cheng Z., Wang J., Li C., Sheng P., Zhang H., Cai M., Guo X., Zhang X., Wang C., Wan J.
Title Overexpression of OsMYB1R1-VP64 fusion protein increases grain yield in rice by delaying flowering time.
Abstract:
VP64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an OsMYB1R1-VP64 fusion protein that enhanced the grain yield of rice cultivar "Kita-ake" by 35%. OsMYB1R1-VP64 regulated grain yield of transgenic plants mainly by extending the vegetative growth stage. Further analysis indicated that OsMYB1R1-VP64 delayed flowering by downregulating expression of heading date 3a (Hd3a) and RICE FLOWERING LOCUST 1 (RFT1) through repression of Early heading date 1 (Ehd1). The expression pattern of OsMYB1R1 was constitutive and rhythmically controlled. OsMYB1R1 protein had transcriptional activation activity and was localized to the nucleus. Our findings suggest that the OsMYB1R1-VP64 construct can be used to increase grain yield in rice. This article is protected by copyright. All rights reserved.
Journal FEBS Lett.
Country China
Volume
Pages
Year 2016
PubMed ID 27545590
PubMed Central ID -
DOI 10.1002/1873-3468.12374
URL -
Relation
Gene EF1 HD3A MYB1R1 RFT1
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase