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| 著者 | Zhang Z., Cheng Z.J., Gan L., Zhang H., Wu F.Q., Lin Q.B., Wang J.L., Wang J., Guo X.P., Zhang X., Zhao Z.C., Lei C.L., Zhu S.S., Wang C.M., Wan J.M. | |
| タイトル | OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice. | |
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Abstract: Cuticular wax, a hydrophobic layer on the surface of all aerial plant organs, has essential roles in plant growth and survival under various environments. Here we report a wax-deficient rice mutant OsHSD1 with reduced epicuticular wax crystals and thicker cuticle membrane. Quantification of the wax components and fatty acids showed elevated levels of very-long-chain fatty acids (VLCFAs) and accumulation of soluble fatty acids in the leaves of the OsHSD1 mutant. We determined the causative gene OsHSD1, a member of the short-chain dehydrogenase reductase family, through map-based cloning. It was ubiquitously expressed and responded to cold stress and exogenous treatments with NaCl or brassinosteroid analogs. Transient expression of OsHSD1-tagged green fluorescent protein revealed that OsHSD1 localized to both oil bodies and endoplasmic reticulum (ER). Dehydrogenase activity assays demonstrated that OsHSD1 was an NAD(+)/NADP(+)-dependent sterol dehydrogenase. Furthermore, OsHSD1 mutation resulted in faster protein degradation, but had no effect on the dehydrogenase activity. Together, our data indicated that OsHSD1 plays a specialized role in cuticle formation and lipid homeostasis, probably by mediating sterol signaling. This work provides new insights into oil-body associated proteins involved in wax and lipid metabolism. |
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| 掲載誌 | Plant Sci. | |
| Country | China | |
| 刊数 | 249 | |
| ページ | 35-45 | |
| 発刊年 | 2016 | |
| PubMed ID | 27297988 | |
| PubMed Central ID | - | |
| DOI | 10.1016/j.plantsci.2016.05.005 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | DRP7 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
