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| 文献 | ||
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| 著者 | Nagano M., Ishikawa T., Fujiwara M., Fukao Y., Kawano Y., Kawai-Yamada M., Shimamoto K. | |
| タイトル | Plasma Membrane Microdomains Are Essential for Rac1-RbohB/H-Mediated Immunity in Rice. | |
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Abstract: Numerous plant defense-related proteins are thought to congregate in plasma membrane microdomains, which consist mainly of sphingolipids and sterols. However, the extent to which microdomains contribute to defense responses in plants is unclear. To elucidate the relationship between microdomains and innate immunity in rice (Oryza sativa), we established lines in which the levels of sphingolipids containing 2-hydroxy fatty acids were decreased by knocking down two genes encoding fatty acid 2-hydroxylases (FAH1 and FAH2) and demonstrated that microdomains were less abundant in these lines. By testing these lines in a pathogen infection assay, we revealed that microdomains play an important role in the resistance to rice blast fungus infection. To illuminate the mechanism by which microdomains regulate immunity, we evaluated changes in protein composition, revealing that microdomains are required for the dynamics of the Rac/ROP small GTPase Rac1 and respiratory burst oxidase homologs (Rbohs) in response to chitin elicitor. Furthermore, FAHs are essential for the production of reactive oxygen species (ROS) after chitin treatment. Together with the observation that RbohB, a defense-related NADPH oxidase that interacts with Rac1, is localized in microdomains, our data indicate that microdomains are required for chitin-induced immunity through ROS signaling mediated by the Rac1-RbohB pathway. |
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| 掲載誌 | Plant Cell | |
| Country | unknown | |
| 刊数 | 28(8) | |
| ページ | 1966-83 | |
| 発刊年 | 2016 | |
| PubMed ID | 27465023 | |
| PubMed Central ID | - | |
| DOI | 10.1105/tpc.16.00201 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | FAH1 FAH2 RAC1 RBOHB RBOHH | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
