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Reference Detail
| Reference | ||
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| Author | Huo C., Zhang B., Wang H., Wang F., Liu M., Gao Y., Zhang W., Deng Z., Sun D., Tang W. | |
| Title | Comparative Study of Early Cold-Regulated Proteins by Two-Dimensional Difference Gel Electrophoresis Reveals a Key Role for Phospholipase Dalpha1 in Mediating Cold Acclimation Signaling Pathway in Rice. | |
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Abstract: To understand the early signaling steps that regulate cold responses in rice, two-dimensional difference gel electrophoresis (2-D DIGE)(1)was used to study early cold-regulated proteins in rice seedlings. Using mass spectrometry, 32 spots, which represent 26 unique proteins that showed an altered expression level within 5 min of cold treatment were identified. Among these proteins, Western blot analyses confirmed that the cellular phospholipase D alpha1 (OsPLDalpha1) protein level was increased as early as 1 min after cold treatment. Genetic studies showed that reducing the expression ofOsPLDalpha1makes rice plants more sensitive to chilling stress as well as cold acclimation increased freezing tolerance. Correspondingly, cold-regulated proteomic changes and the expression of the cold-responsive C repeat/dehydration-responsive element binding 1 (OsDREB1) family of transcription factors were inhibited in thepldalpha1mutant. We also found that the expression ofOsPLDalpha1is directly regulated by OsDREB1A. This transcriptional regulation ofOsPLDalpha1could provide positive feedback regulation of the cold signal transduction pathway in rice. OsPLDalpha1 hydrolyzes phosphatidylcholine to produce the signal molecule phosphatidic acid (PA). By lipid-overlay assay, we demonstrated that the rice cold signaling proteins, MAP kinase 6 (OsMPK6) and OsSIZ1, bind directly to PA. Taken together, our results suggest that OsPLDalpha1 plays a key role in transducing cold signaling in rice by producing PA and regulatingOsDREB1s' expression by OsMPK6, OsSIZ1, and possibly other PA-binding proteins. |
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| Journal | Mol. Cell Proteomics | |
| Country | China | |
| Volume | 15(4) | |
| Pages | 1397-411 | |
| Year | 2016 | |
| PubMed ID | 26747563 | |
| PubMed Central ID | 4824863 | |
| DOI | 10.1074/mcp.M115.049759 | |
| URL | - | |
| Relation | ||
| Gene | DREB1A PLDalpha1 SIZ1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
