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Reference Detail
| Reference | ||
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| Author | Yang X., Gong P., Li K., Huang F., Cheng F., Pan G. | |
| Title | A single cytosine deletion in the OsPLS1 gene encoding vacuolar-type H+-ATPase subunit A1 leads to premature leaf senescence and seed dormancy in rice. | |
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Abstract: leaf senescence is a programmed developmental process orchestrated by many factors, but its molecular regulation is not yet fully understood. In this study, a novel Oryza sativa premature leaf senescence mutant (OsPLS1) was examined. Despite normal development in early seedlings, the OsPLS1 mutant leaves displayed lesion-mimics and early senescence, and a high transpiration rate after tillering. The mutant also showed seed dormancy attributable to physical (defect of micropyle structure) and physiological (abscisic acid sensitivity) factors. Using a map-based cloning approach, we determined that a cytosine deletion in the OsPLS1 gene encoding vacuolar H(+)-ATPase subunit A1 (VHA-A1) underlies the phenotypic abnormalities in the OsPLS1 mutant. The OsPSL1/VHA-A1 transcript levels progressively declined with the age-dependent leaf senescence in both the OsPLS1 mutant and its wild type. The significant decrease in both OsPSL1/VHA-A1 gene expression and VHA enzyme activity in the OsPLS1 mutant strongly suggests a negative regulatory role for the normal OsPLS1/VHA-A1 gene in the onset of rice leaf senescence. The OsPLS1 mutant featured higher salicylic acid (SA) levels and reactive oxygen species (ROS) accumulation, and activation of signal transduction by up-regulation of WRKY genes in leaves. Consistent with this, the OsPLS1 mutant exhibited hypersensitivity to exogenous SA and/or H2O2 Collectively, these results indicated that the OsPSL1/VAH-A1 mutation played a causal role in premature leaf senescence through a combination of ROS and SA signals. To conclude, OsPLS1 is implicated in leaf senescence and seed dormancy in rice. |
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| Journal | J. Exp. Bot. | |
| Country | China | |
| Volume | 67(9) | |
| Pages | 2761-76 | |
| Year | 2016 | |
| PubMed ID | 26994476 | |
| PubMed Central ID | 4861022 | |
| DOI | 10.1093/jxb/erw109 | |
| URL | - | |
| Relation | ||
| Gene | KAT1 PAL PAL1 PAL1 PLS1 RCCR1 SGR VHA-A2 WRKY107 WRKY42 WRKY53 WRKY6 WRKY71 WRKY72 WRKY77 WRKY79 _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
