Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Liu J., Chen X., Liang X., Zhou X., Yang F., Liu J., He S.Y., Guo Z. | |
| Title | Alternative splicing of rice WRKY62 and WRKY76 transcription factor genes in pathogen defense. | |
|
Abstract: The WRKY family of transcription factors (TFs) function as transcriptional activators or repressors in various signaling pathways. In this study we discovered that two IIa subfamily of WRKY genes, OsWRKY62 and OsWRKY76, undergo constitutive and inducible alternative splicing. The full-length OsWRKY62.1 and OsWRKY76.1 proteins formed homo- and hetero-complexes, but the heterocomplex dominates in the nuclei when analyzed in Nicotiana benthamiana leaves. Transgenic overexpression of OsWRKY62.1 and OsWRKY76.1 enhanced susceptibility to the rice blast fungus Magnaporthe oryzae SZ and the rice leaf blight bacterium Xanthomonas oryzae pv. oryzae J18, whereas RNAi and loss-of-function knockout plants exhibited elevated resistance. The dsOW62/76 and knockout lines of OsWRKY62 and OsWRKY76 also showed greatly increased expressions of defense-related genes and accumulation of phytoalexins. Unlike other WRKY genes analyzed so far, we observed prominent alternative transcripts for OsWRKY62.1, OsWRKY76.1 and, most interestingly, the ratio of full-length vs. truncated transcripts changed in dsOW62/76 plants as well as in response to pathogen infection. The short OsWRKY62.2 and OsWRKY76.2 proteins could interact each other and with full-length proteins. OsWRKY62.2 showed a reduced repressor activity in planta and two sequence determinants required for the repressor activity were identified in the N-terminus of OsWRKY62.1. The N-termini of OsWRKY62 and OsWRKY76 splice variants also showed reduced binding to the canonical W box motif. These results enhance our understanding of the DNA binding property, the repressor sequence motifs and the negative feedback regulation of the IIa subfamily of WRKYs and provide the first evidence for alternative splicing of WRKY TFs. |
||
| Journal | Plant Physiol. | |
| Country | unknown | |
| Volume | ||
| Pages | ||
| Year | 2016 | |
| PubMed ID | 27208272 | |
| PubMed Central ID | - | |
| DOI | 10.1104/pp.15.01921 | |
| URL | - | |
| Relation | ||
| Gene | CPS2 CPS4 CYP71Z7 MAS PAL1 PR10A PR1A WRKY62 WRKY76 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
