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| 著者 | Nakamura Y., Kubo A., Shimamune T., Matsuda T., Harada K., Satoh H. | |
| タイトル | Correlation between activities of starch debranching enzyme and alpha-polyglucan structure in endosperms of sugary-1 mutants of rice | |
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Abstract: The biochemical lesion of the sugary-1 mutation was examined in five different mutants of rice with varying phenotypes but with mutations at the same locus. The cells in the inner part of the endosperm of all mutants tested contained phytoglycogen instead of starch, while the cells located in the outer part of the endosperm tissue from some mutants were filled with numerous starch granules. The molecular size of phytoglycogen was markedly smaller than that of amylopectin as measured by Sephacryl S-1000 chromatography. Analysis of the distribution of alpha-1,4 chain lengths revealed that in phytoglycogen the number of A-chains dramatically increased, while long B chains with DP ≥ 37 remarkably decreased or were almost absent, which resulted in the disappearance of the cluster structure. The results suggest that changes in the balance of enzymic activities induced by the mutations brought about a drastic alteration in polyglucan structure and the shape of the polyglucan granule. The greater the extent of phytoglycogen regions in su1 endosperm tissues became, the greater was the phytoglycogen content, and the greater the reduction in the activity of starch debranching enzyme, a type of enzyme referred to as R-enzyme (RE), limit dextrinase or pullulanase. Immunoblot analysis showed that the reduction in RE activity was due to a decrease in the amount of RE protein, and that the reduction in RE was specific since proteins of starch-branching enzymes I and IIa and ADP-glucose pyrophosphorylase were not markedly affected by su1 mutations. The proportion of starch region to the whole endosperm tissue of various su1 mutants was correlated with the RE activity in these endosperms. The results strongly suggest that the reduction in RE activity is involved in the su1 phenotype and that the enzyme plays an essential role in determining the fine structure of the amylopectin molecule. |
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| 掲載誌 | The Plant Journal | |
| Country | Japan | |
| 刊数 | 12(1) | |
| ページ | 143-153 | |
| 発刊年 | 1997 | |
| PubMed ID | - | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | PUL SUG | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
