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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zhang X., Sun J., Cao X., Song X. | |
| Title | Epigenetic Mutation of RAV6 Affects leaf angle and Seed Size in Rice. | |
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Abstract: Heritable epigenetic variants of genes, termed epialleles, can broaden genetic and phenotypic diversity in eukaryotes. Epialleles may also provide a new source of beneficial traits for crop breeding, but very few epialleles related to agricultural traits have been identified in crops. Here, we identified Epi-RAV6, a gain-of-function epiallele of rice (Oryza sativa) RELATED TO ABSCISIC ACID INSENSITIVE3 (ABI3)/VIVIPAROUS1 (VP1) 6 (RAV6), which encodes a B3 DNA-binding domain-containing protein. The Epi-RAV6 plants show larger lamina inclination and smaller grain size; these agronomically important phenotypes are inherited in a semidominant manner. We did not find nucleotide sequence variation of RAV6. Instead, we found hypomethylation in the promoter region of RAV6, which caused ectopic expression of RAV6 in Epi-RAV6 plants. Bisulfite analysis revealed that cytosine methylation of four CG and two CNG loci within a continuous 96-bp region plays essential roles in regulating RAV6 expression; this region contains a conserved miniature inverted repeat transposable element transposon insertion in cultivated rice genomes. Overexpression of RAV6 in the wild type phenocopied the Epi-RAV6 phenotype. The brassinosteroid (BR) receptor BR INSENSITIVE1 and BR biosynthetic genes EBISU DWARF, DWARF11, and BR-DEFICIENT DWARF1 were ectopically expressed in Epi-RAV6 plants. Also, treatment with a BR biosynthesis inhibitor restored the leaf angle defects of Epi-RAV6 plants. This indicates that RAV6 affects rice leaf angle by modulating BR homeostasis and demonstrates an essential regulatory role of epigenetic modification on a key gene controlling important agricultural traits. Thus, our work identifies a unique rice epiallele, which may represent a common phenomenon in complex crop genomes. |
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| Journal | Plant Physiol. | |
| Country | unknown | |
| Volume | 169(3) | |
| Pages | 2118-28 | |
| Year | 2015 | |
| PubMed ID | 26351308 | |
| PubMed Central ID | 4634063 | |
| DOI | 10.1104/pp.15.00836 | |
| URL | - | |
| Relation | ||
| Gene | AP2/EREBP127 AP2/EREBP129 AP2/EREBP77 AP2/EREBP96 BRD1 D11 D2 D61 NGA1 NGA4 NGA5 RAP2.8 RAV6 RAVL1 WRKY123 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
