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Author Du W., Cheng J., Cheng Y., Wang L., He Y., Wang Z., Zhang H.
Title Physiological characteristics and related gene expression of after-ripening on seed dormancy release in rice.
Abstract:
After-ripening is a common method used for dormancy release in rice. In this study, the rice variety Jiucaiqing (Oryza sativa L. subsp. japonica) was used to determine dormancy release following different after-ripening times (1, 2 and 3 months). Germination speed, germination percentage and seedling emergence increased with after-ripening; more than 95% germination and 85% seedling emergence were observed following 1 month of after-ripening within 10 days of imbibition, compared with <45% germination and 20% seedling emergence in freshly harvested seed. Hence, 3 months of after-ripening could be considered a suitable treatment period for rice dormancy release. Dormancy release by after-ripening is mainly correlated with a rapid decline in ABA content and increase in IAA content during imbibition. Subsequently, GA1 /ABA, GA7 /ABA, GA12 /ABA, GA20 /ABA and IAA/ABA ratios significantly increased, while GA3 /ABA, GA4 /ABA and GAs/IAA ratio significantly decreased in imbibed seeds following 3 months of after-ripening, thereby altering alpha-amylase activity during seed germination. Peak alpha-amylase activity occurred at an earlier germination stage in after-ripened seeds than in freshly harvested seeds. Expression of ABA, GA and IAA metabolism genes and dormancy-related genes was regulated by after-ripening time upon imbibition. Expression of OsCYP707A5, OsGA2ox1, OsGA2ox2, OsGA2ox3, OsILR1, OsGH3-2, qLTG3-1 and OsVP1 increased, while expression of Sdr4 decreased in imbibed seeds following 3 months of after-ripening. Dormancy release through after-ripening might be involved in weakening tissues covering the embryo via qLTG3-1 and decreased ABA signalling and sensitivity via Sdr4 and OsVP1.
Journal Plant Biol (Stuttg)
Country China
Volume
Pages
Year 2015
PubMed ID 26205956
PubMed Central ID -
DOI 10.1111/plb.12371
URL -
Relation
Gene ABA8OX1 GA2OX1 GH3-8 HYPRP5 NCED1 NCED3 SDR4 VP1 _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase